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Determination of a molecular torsional angle in the metarhodopsin-I photointermediate of rhodopsin by double-quantum solid-state NMR

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Abstract

We present a solid-state NMR study of metarhodopsin-I, the pre-discharge intermediate of the photochemical signal transduction cascade of rhodopsin, which is the 41 kDa integral membrane protein that triggers phototransduction in vertebrate rod cells. The H-C10-C11-H torsional angles of the retinylidene chromophore in bovine rhodopsin and metarhodopsin-I were determined simultaneously in the photo-activated membrane-bound state, using double-quantum heteronuclear local field spectroscopy. The torsional angles were estimated to be |φ| = 160 ± 10° for rhodopsin and φ= 180 ± 25° for metarhodopsin-I. The result is consistent with current models of the photo-induced conformational transitions in the chromophore, in which the 11-Z retinal ground state is twisted, while the later photointermediates have a planar all-E conformation.

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Authors and Affiliations

  1. Physical Chemistry Division, Arrhenius Laboratory, Stockholm University, S-10691, Stockholm, Sweden

    X. Feng, P.J.E. Verdegem, M. Edén, D. Sandström, Y.K. Lee & M.H. Levitt

  2. Department of Biochemistry, Institute of Cellular Signalling, University of Nijmegen, P.O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands

    P.H.M. Bovee-Geurts & W.J. de Grip

  3. Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, P.O. Box 9502, NL-2300 RA, Leiden, The Netherlands

    J. Lugtenburg & H.J.M. de Groot

Authors
  1. X. Feng
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  2. P.J.E. Verdegem
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  3. M. Edén
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  4. D. Sandström
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  5. Y.K. Lee
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  6. P.H.M. Bovee-Geurts
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  7. W.J. de Grip
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  8. J. Lugtenburg
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  9. H.J.M. de Groot
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  10. M.H. Levitt
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Feng, X., Verdegem, P., Edén, M. et al. Determination of a molecular torsional angle in the metarhodopsin-I photointermediate of rhodopsin by double-quantum solid-state NMR. J Biomol NMR 16, 1–8 (2000). https://doi.org/10.1023/A:1008377231625

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