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Survey and analysis of commercial cellulase preparations suitable for biomass conversion to ethanol

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Abstract

Commercial cellulase enzymes have been used in the food, detergent, and textile industries, and are potentially effective for processing biomass feedstocks. A survey was undertaken to identify major manufacturers/distributors of cellulases in the USA and to evaluate 13 representative commercial preparations for enzyme activity, protein concentration, and chemical composition. Samples were subjected to activity measurements using filter paper, carboxymethylcellulose, cellobiose, and p-nitrophenyl-β-d-glucopyranoside as substrates. To ascertain the microbial origin of the commercial preparations, Western blots utilizing monoclonal antibodies specific for Trichoderma reesei CBH I and Aspergillus niger β-d-glucosidase were developed. Eleven of the cellulases tested were of T. reesei or T. viride origin and two were from A. niger.

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Authors and Affiliations

  1. National Renewable Energy Laboratory, Biotechnology Center for Fuels and Chemicals, Golden, CO, 80401, USA ; fax

    R.A. Nieves, C.I. Ehrman, W.S. Adney, R.T. Elander & M.E. Himmel

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  1. R.A. Nieves
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  2. C.I. Ehrman
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  3. W.S. Adney
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  4. R.T. Elander
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  5. M.E. Himmel
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Nieves, R., Ehrman, C., Adney, W. et al. Survey and analysis of commercial cellulase preparations suitable for biomass conversion to ethanol. World Journal of Microbiology and Biotechnology 14, 301–304 (1997). https://doi.org/10.1023/A:1008871205580

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  • DOI: https://doi.org/10.1023/A:1008871205580

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