Abstract
A novel carrier obtained from spent grains, a brewing by-product, was used for brewing yeast immobilisation in a continuous bubble-column reactor. The multiple-layer cell adhesion to the carrier particles resulted in a maximum cell load of 430 mg dry cell g−1 dry carrier (d.c.). After 120 h of reactor operation, the cell load of DEAE-modified carrier was below 40 mg dry cell g−1 d.c. while the values for non-modified carrier reached at least 100 mg dry cell g−1 d.c. The changes in substrate composition on the rate of yeast attachment and on its stability were also studied.
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Brányik, T., Vicente, A., Machado Cruz, J. et al. Spent grains – a new support for brewing yeast immobilisation. Biotechnology Letters 23, 1073–1078 (2001). https://doi.org/10.1023/A:1010558407475
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DOI: https://doi.org/10.1023/A:1010558407475