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Gelatin nanoparticles produced by a simple W/O emulsion as delivery system for methotrexate

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Abstract

Biodegradable hydrophilic gelatin nanoparticles, containing different initial amounts of methotrexate (MTX), were prepared using a simple solvent evaporation technique based on a single water-in-oil emulsion and stabilized by the use of glutaraldehyde as cross-linking agent. The effects of several parameters on particle size, drug encapsulation efficiency and drug release were investigated. Size and shape of the nanoparticles were examined by scanning electron microscopy. The release of MTX was monitored in vitro and the mechanism of release was studied. Particles with a mean diameter of 100–200 nm were produced, which were able to release MTX following a diffusion-controlled mechanism of release. It was observed that the initial amount of MTX used for sample loading did not have any effect on the pattern of release, while it affected the amount of drug entrapped into the nanoparticles and also both the release rate and the total amount of drug released.

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Authors and Affiliations

  1. Department of Chemical Engineering, University of Pisa, Via Diotisalvi 2, 56126, Pisa, Italy

    Maria Grazia Cascone & Luigi Lazzeri

  2. Department of Bioorganic Chemistry and Biopharmacy, University of Pisa, Via Bonanno 33, 56126, Pisa, Italy

    Claudia Carmignani

  3. Department of Biochemistry and Pharmacology, Shaanxi Institute of Pharmaceutical Industry, Changlezhong Road 9, 710032, Xian, P.R. China

    Zhouhai Zhu

Authors
  1. Maria Grazia Cascone
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  2. Luigi Lazzeri
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  3. Claudia Carmignani
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  4. Zhouhai Zhu
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Correspondence to Maria Grazia Cascone.

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Cascone, M.G., Lazzeri, L., Carmignani, C. et al. Gelatin nanoparticles produced by a simple W/O emulsion as delivery system for methotrexate. Journal of Materials Science: Materials in Medicine 13, 523–526 (2002). https://doi.org/10.1023/A:1014791327253

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  • DOI: https://doi.org/10.1023/A:1014791327253

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