Abstract
One hundred and twenty-three fungal isolates were obtained from 225 root segments of eggplants, melon, tomato, strawberry and Chinese cabbage, grown as bait plants in a mixed soil made up of samples from different fields in Shizuoka, Japan. Isolates belonging to Mycelium radicis atrovirens (MRA), including Phialocephala fortinii, were the most prevalent in all the five bait plants. Eleven of the 123 isolates, after being inoculated onto axenically reared eggplant seedlings, almost completely suppressed the pathogenic effects of a post-inoculated, virulent strain of Verticillium dahliae. Seven of these 11 isolates had come from the roots of eggplant and included Heteroconium chaetospira, P. fortinii, and unidentified species of Fusarium, Penicillium, Trichoderma and MRA. P. fortinii, H. chaetospira, a non-sporulating isolate with white mycelium (SWM) and MRA were easily reisolated from root segments. Hyphae of H. chaetospira, P. fortinii and SWM colonized the root tissues of eggplant without causing apparent pathogenic symptoms. The mechanisms by which these endophytes confer resistance to infection by V. dahliae are unknown but the effectiveness of these fungi in a laboratory setting indicates that they have potential as biocontrol agents and merit further investigation.
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References
Addy HD, Hambleton S and Currah RS (2000) Distribution and molecular characterization of the root endophyte, Phialocephala fortinii an environmental gradient in the boreal forest of Alberta. Mycological Research 104: 1213–1221
Bletsos FA, Thanassoulopoulos CC and Roupakias DG (1999) Water stress and Verticillium wilt severity on eggplant (Solanum melongena L.). Journal of Phytopathology 147: 243–248
Currah RS, Tsuneda A and Murakami S (1993) Morphology and ecology of Phialocephala fortinii in roots of Rhododendron brachycarpum. Canadian Journal of Botany 71: 1639–1644
Currah RS, Sigler L and Hambleton S (1987) New records and new taxa of fungi from the mycorrhizae of terrestrial orchids of Alberta. Canadian Journal of Botany 65: 2473–2482
De Melo IS and De Costa CP (1987) Biological control of wilt of eggplant caused by Verticillium albo-atrum. Anals da Escola Superior de Agricultura' Luiz de Queiroz' 44: 1353–1364
Domsch KH, Gams W and Anderson TH (1993). Compendium of Soil Fungi. Academic Press, London.
Ellis MB (1976) More Dematiaceous Hyphomycetes. Commonwealth Mycological Institute.
Fernando AA and Currah RS (1996) A comparative study of the effects of the root endophytes Leptodontidium orchidicola and Phialocephala fortinii (Fungi Imperfecti) on the growth of some subalpine plants in culture. Canadian Journal of Botany 74: 1071–1078
Haselwandter K and Read DJ (1982) The significance of a root-fungus association in two Carex species of high-alpine plant communities. Oecologia 53: 352–354
Huisman OC and Gerik JS (1989) Dynamics of colonization of plant roots by Verticillium dahliae and other fungi. In: Tjamos EC and Beckman CH (eds) Vascular wilt disease of plants, (pp 1–17) Springer-Verlag, Berlin, Heidelberg
Jarvis WR (1993) Managing diseases in greenhouse crops. American Phytopathological Society Press, St. Paul, Minnesota
Jumpponen AT and Trappe JM (1998) Dark septate endophytes: a review of facultative biotrophic root-colonizing fungi. New Phytologist 140: 295–310
Marois JJ, Johnston SA, Dunn MT and Papavizas GC (1982) Biological control of Verticillium wilt of eggplant in the field. Plant Disease 66: 1166–1168
Matsushima T (1975) Icones microfungorum a Matsushima lectorum. Kobe, Japan. 209 pp and 415 plates
Narisawa K, Ohki T and Hashiba T (2000) Suppression of clubroot and Verticillium yellows in Chinese cabbage in the field by the endophytic fungus, Heteroconium chaetospira. Plant Pathology 49: 141–146
Narisawa K, Tokumasu S and Hashiba T (1998) Suppression of clubroot formation in Chinese cabbage by root endophytic fungus, Heteroconium chaetospira. Plant Pathology 47: 210–216
O'Dell TE, Massicotte HB and Trappe JM (1993) Root colonization of Lupinus latifolius Agardh. and Pinus contorta Dougl. by Phialocephala fortinii Wang & Wilcox. New Phytologist 124: 93–100
Schulz B, Römmert A-K, Dammann U, Aust H-J and Strack D (1999) The endophyte–host interaction: a balanced antagonism? Mycological Research 103: 1275–1283
Stoyke G and Currah RS (1991) Endophytic fungi from the mycorrhizae of alpine ericoid plants. Canadian Journal of Botany 69: 347–352
Tjamos EC (2000). Strategies in developing methods and applying techniques for the biological control of Verticillium dahliae. American Phytopathological Society Press, St. Paul, Minnesota
Turhan G (1981) A new race of Streptomyces ochraceiscleroticus in the biological control of some soil-borne plant pathogens. II. In vivo studies on the possibilities of using C/2-9 against some important diseases. Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz 88: 422–434
Yamaguchi K, Sano T, Arita M and Takahashi M (1992) Biocontrol of Fusarium wilt of tomato and Verticillium wilt of eggplant by non-pathogenic Fusarium oxysporum MT0062. Annals of the Phytopathological Society of Japan 58: 188–194
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Narisawa, K., Kawamata, H., Currah, R.S. et al. Suppression of Verticillium Wilt in Eggplant by Some Fungal Root Endophytes. European Journal of Plant Pathology 108, 103–109 (2002). https://doi.org/10.1023/A:1015080311041
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DOI: https://doi.org/10.1023/A:1015080311041