Skip to main content
SpringerLink
Log in

In vitro production and identification of autotetraploids of Scutellaria baicalensis

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

The roots of Scutellaria baicalensis are a major traditional Chinese medicine. We report research on induction, characteristics and chemical analysis of polyploid plants of S. baicalensis. Immersing calluses in 0.2% colchicine solution for 12 h prior to culture induced a high number of tetraploid plants. The induction rate reached as high as 40% of treated calluses. More than 50 lines of tetraploid plants were obtained. All tetraploid plants showed typical polyploidy characteristics. Twenty selected tetraploid lines were transferred to the field for determination of morphological characteristics and for chemical assays. Seven elite lines have been selected for further selection and breeding into new varieties for commercial production.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Chen BJ, Gao SL & Bian YY (2000) The induction of autotetraploids of Scutellaria baicalensis Georgi by tissue culture. J. Plant Res. Environ. 9: 9–11

    Google Scholar 

  • Gao SL, Zhu DN, Cai ZH & Xu DR (1996) Autotetraploid plants from colchicine-treated bud culture of Salvia miltiorrhiza Bge. Plant Cell Tiss. Org. Cult. 47: 73–77

    Google Scholar 

  • Jackson BP & Rowson JM (1953) Alkaloid biogenesis in tetraploid Stramonium. J. Pharm. Pharmacol. 5: 778–793

    Google Scholar 

  • Lewis WH (1980) Polyloidy: Biological Relevance. (pp 180–182) Plenum Press, New York

    Google Scholar 

  • Morimoto S, Harioka T & Shoyama Y (1995) Purification and characterization of flavone-specific beta-glucuronidase from callus culture of Scutellaria baicalensis Georgi. Planta 195: 535–540

    Google Scholar 

  • Pei XS (1985) Polyploidy Induction and Breeding. (pp 95–98) Shanghai Science and Technology Press

  • Rowson JM(1949) Increased alkaloid contents of induced polyploid of Datura. Nature 154: 81–82

    Google Scholar 

  • Yamamoto H, Chatani N & Kitayama A (1986) Flavonoid production in Scutellaria baicalensis callus cultures. Plant Cell Tiss. Org. Cult. 5: 219–222

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Genetics and Breeding, China Pharmaceutical University, Ji Xiang An, Nanjing, P.R. China, 210038

    S. L. Gao, B. J. Chen & D. N. Zhu

Authors
  1. S. L. Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. J. Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. N. Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gao, S.L., Chen, B.J. & Zhu, D.N. In vitro production and identification of autotetraploids of Scutellaria baicalensis . Plant Cell, Tissue and Organ Culture 70, 289–293 (2002). https://doi.org/10.1023/A:1016577002039

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1016577002039

Search

Navigation