Skip to main content
SpringerLink
Log in

Haploidy breeding and mutagenesis for drought tolerance in wheat

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Several intraspecific crosses between known drought tolerant wheat varieties and stable high yielding recombinants were made with the objective to develop improved cultivars for the moisture stressed rainfed areas of Pakistan. Five of these crosses were selected for further creation of useful mutations through the application of low doses of gamma rays and development of doubled haploids through anther culture. Anther culture response of the selected irradiated F1 generations was studied on liquid and solid induction media. The highest number of calli among almost all crosses was produced on Potato-2induction medium. All the crosses varied greatly in response to callus induction and maximum calli (75%) were obtained from Lyl-73/vee’s’ cross. Similarly, genotypic differences were found for green vs. albino regenerants. The highest number of green plantlets (12.1%) was recorded for Lu-26/3062. From the developed doubled haploid population 25 DH-mutants were initially selected and nine lines were finally included in multi-locational field tests. Two DH-mutants (i.e. DHML-50 and DHML-9) have potential for better grain yield, earliness, disease resistance and moisture stress tolerance.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Chuang, C.C., T.W. Ouyang, H. Chia, S.M.M. Chou & C.K. Ching, 1978. A set of potato media for wheat anther culture. In: Proc. Symp. Plant Tissue Culture, pp. 51–56. Science Press, Peking.

    Google Scholar 

  • Ding, X.L., D.J. Luckett & N.L. Darvey, 1991. Low dose gamma irradiation promotes wheat anther culture response. Aust J Bot 39: 467–474.

    Article  Google Scholar 

  • Henry, Y., J. De-Buyser, M. Amssa & G. Taleb, 1982. In vitro androgenesis in winter wheat breeding. In: A. Fujiwara (Ed.), Proc. 5th Intl. Cong. Plant Tissue and Cell Culture, pp. 569–570. Abe Photo Printing Co., Tokyo.

    Google Scholar 

  • Hu, D., 1996. A series of Jinghua winter wheat varieties developed by anther culture. In: Proc. 8th Assembly Wheat Breeding Society of Australia, pp. 144–147.

  • Hu, H., 1986. Wheat: improvement through anther culture. In: Y.P.S. Bajaj (Ed.), Biotechnology in Agriculture and Forestry 2, pp. 55–72. Springer Verlag, Berlin.

    Google Scholar 

  • Konzak, C.F. & H. Zhou, 1991. Anther culture methods for doubled haploid production in wheat. Proc. FAO/IAEA Meeting, Katowice, Maluszynski, M. & Z. Barabas (Eds.), Cereal Res Commun 19(1-2): 147–164.

  • Kramer, P.J., 1980. Drought stress and the origin of adaptations. In: N.C. Turner & P.J. Kramer (Eds.), Adaptation of plants to water and high temperature stress, pp. 7–20. JohnWiley and Sons, New York.

    Google Scholar 

  • Ouyang, J.W., 1986. Induction of pollen plants in Triticum aestivum. In: H. Hu & H. Yang (Eds.), Haploids of Higher Plants In Vitro, pp. 26–41. China Academic Publishers, Springer-Verlag, Beijing, Berlin.

    Google Scholar 

  • Ouyang, J.W., S.M. Zhou & S.E. Jia, 1983. The response of anther culture to culture temperature in Triticum aestivum. Theor Appl Genet 66: 101–109.

    Article  Google Scholar 

  • SAS Institute, 1990. SAS/STAT guide for personal computer, version 6.04. SAS Institute, Cary.

    Google Scholar 

  • Singh, T.N., D Aspinall, L.G. Paleg & S.F. Bogges, 1973a. Stress metabolism: II. Changes in proline concentration in excised plant tissues. Aust J Bio Sci 26(57): 63.

    Google Scholar 

  • Singh, T.N., L.G. Paleg & D. Aspinall, 1973b. Stress metabolism: I. Nitrogen metabolism and growth in barley during water stress. Aust J Bio Sci 26: 45–56.

    CAS  Google Scholar 

  • Stewart, C.R. & A.D. Hanson, 1980. Proline accumulation as a methabolic response to water stress. In: N.C. Turner & P.J. Kramer (Eds.), Adaptation of plants to water and high temperature stress, pp. 173–189. John Wiley and Sons, New York.

    Google Scholar 

  • Tan, B.H. & G.M. Halloran, 1980. Variation and correlations of proline accumulation in spring wheat cultivars. Crop Sci 22: 459–463.

    Article  Google Scholar 

  • Zapata, F.J. & R.R. Aldemita, 1986. Induction of salt tolerance in high yielding rice varieties through mutagenesis and anther culture. In: M. Maluszynski (Ed.), Current Options for Cereal Improvement, pp. 193–202. Kluwer Academic Publisher, Dordrecht.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Nuclear Institute for Food and Agriculture (NIFA), Tarnab-Peshawar, P.O. Box 446, Pakistan

    A.J. Khan, S. Hassan, M. Tariq & T. Khan

Authors
  1. A.J. Khan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Hassan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Tariq
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. Khan
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Khan, A., Hassan, S., Tariq, M. et al. Haploidy breeding and mutagenesis for drought tolerance in wheat. Euphytica 120, 409–414 (2001). https://doi.org/10.1023/A:1017598202368

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1017598202368

Search

Navigation