Abstract
Protein release from degradable polymer matrices, composed of poly(L-lactic acid) and its blends with Pluronic surfactant, was investigated with and without the aqueous coating of an adsorptive water-soluble polymer, polyethyleneimine (PEI). PEI is a highly branched cationic polymer containing primary, secondary, and tertiary amino groups in its backbone. The treatment of PEI for PLA/Pluronic blend films exhibited a remarkable decrease in the “burst” release of protein at an initial stage and a significant extension in the protein release period. Protein release profiles could be controlled by varying PEI treatment time and its concentration. Our results suggest that PEI diffuses into the polymer matrices and crosslinks protein molecules by ionic interactions. Such a PEI–protein network near the surface region of matrix may act as a diffusional barrier for further release of protein molecules.
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REFERENCES
R. Langer. New methods of drug delivery. Science 249:1527–1533 (1990).
W. Sadée. Protein drugs: A revolution in therapy? Pharm. Res. 3:3–6 (1986).
C. G. Pitt. The controlled parenteral delivery of polypeptides and proteins. Int. J. Pharm. 59:173–196 (1990).
S. Cohen, T. Yoshioka, M. Lucarelli, L. H. Hwang, and R. Langer. Controlled delivery systems for proteins based on poly(lactic/glycolic acid) microspheres. Pharm. Res., 8:713–720 (1991).
R. Langer, L. Brown, and E. Edelman. Controlled release and magnetically modulated release systems for macromolecules. Methods Enzymol. 112:399–422 (1985).
A. K. Kwong, S. Chou, A. M. Sun, M. V. Sefton, and M. F. A. Goosen. In vitro and in vivo release of insulin from poly(lactic acid) microbeads and pellets. J. Contr. Rel. 4:47–62 (1986).
T. G. Park, S. Cohen, and R. Langer. Poly(L-lactic acid)/Pluronic™ blends: Characterization of phase separation behavior, degradation, morphology, and as protein releasing matrices. Macromolecules, in press (1991).
N. Marcotte, A. Polk, and M. F. A. Goosen. Kinetics of protein diffusion from a poly(D,L-lactide) reservoir system. J. Pharm. Sci. 79:407–410 (1990).
P. Molyneux. Water Soluble Synthetic Polymers: Properties and Behavior, Vol. 1, CRC Press, Boca Raton, FL, 1984, pp. 62–65.
R. R. Burgess and J. J. Jendrisak. A procedure for the rapid, large scale purification of Escherichia coli DNA-dependent RNA polymerase involving Polyamin P precipitation and DNA-cellulose chromatography. Biochemistry 14:4634–4638 (1975).
H. Moroson and M. Rotman. Biomedical applications of polycations. In A. Rembaum and E. Selegny (eds.), Polyelectrolyte and Their Applications, Reidel, Dordrecht, Holland, 1975, pp. 187–195.
C. C. Heuck and G. Schlierf. Beta-lipoprotein cholesterol quantification with polycations. Clin. Chem. 23:536–540 (1977).
D. A. Tomalia, A. M. Naylor, and W. A. Goddard III. Starburst dendrimers: Molecular-level control of size, shape, surface chemistry, topology, and flexibility from atoms to macroscopic matter. Angew. Chem. Int. Ed. Engl. 29:138–175 (1990).
J. P. Tam. Synthetic peptide vaccine design: Synthesis and properties of a high-density multiple-antigenic peptide system. Proc. Natl. Acad. Sci. 85:5409–5413 (1988).
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Park, T.G., Cohen, S. & Langer, R. Controlled Protein Release from Polyethyleneimine-Coated Poly(L-lactic Acid)/Pluronic Blend Matrices. Pharm Res 9, 37–39 (1992). https://doi.org/10.1023/A:1018971525301
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DOI: https://doi.org/10.1023/A:1018971525301