Abstract
Menadione-catalyzed H2O2 production by viable animal cells was proportional to the viable cell number, and H2O2 production decreased with increasing cytotoxic effects after the incubation of cells with cytotoxic compounds. The cytotoxic effects of food additives, pesticides, antibiotics, heavy metals, phytotoxins, mycotoxins, and marine toxins were estimated using the above test employingNIH/3T3 and Neuro-2a cells. Synergistic effects of the toxin mixture were observed and acute cytotoxicity detected 1 h after the incubation of cells with toxins. This menadione-catalyzed H2O2production assay is rapid and simple compared to other popular cytotoxicity tests such as the MTT reduction assay and Neutral red inclusion test, requiring4 h. The menadione-catalyzed H2O2 production assay is expected to be a useful food safety test for rapidly detecting toxic compounds having a basic cytotoxic effect on common animal cells.
Similar content being viewed by others
References
Ecobichon D.J. 1996. Toxic effects of pesticides. In: Klaassen C.D., Amdur M.O. and Doull J. (eds), Casarett & Doull's Toxicology. McGraw-Hill, New York, pp. 643–689.
Ekwall B. 1983. Correlation between cytotoxicity in vitro and LD50values. Acta. Pharmacol. et. Toxicol. 52: 80–99.
Goyer R.A. 1996. Toxic effects of metals. In: Klaassen C.D., Amdur M.O. and Doull J. (eds), Casarett & Doull's Toxicology. McGraw-Hill, New York, pp. 738.
Ikemoto H. and Ueda S. 1994. Manual of Antibiotics and Infectious Disease. Hirokawa Publishing Co., Tokyo.
Jaeger A., Jehl F. and Flesch F. 1993. Kinetics of amatoxins in human poisoning: Therapeutic implication. Clin. Toxicol. 31: 68–80.
Kao C.Y. and Fuhrman F.A. 1963. Pharmacological studies on tarichatoxin, a potent neurotoxin. J. Pharmacol. Exp. Ther. 140: 31.
Kenkens E.A.J., de Vrije T., van den Boom C., de Waard P., Plasman H.H., Thiel F. et al. 1995. Molecular basis of glycoal-kaloid induced membrane disruption. Biochim. Biophys. Acta. 1240: 216–228.
Konemann W.H. and Pieters M.N. 1996. Confusion of concepts in mixture toxicology. Food and Chem. Toxicol. 34: 1025–1031.
Kotsonis F.N., Burdock G.A. and Flamm W.G. 1996. Food toxicology. In: Klaassen C.D., Amdur M.O. and Doull J. (eds), Casarett & Doull's Toxicology. McGraw-Hill, New York, pp. 922.
Manger R.L., Leja L.S., Lee S.Y., Hungerford J.M. and Wekell M.M. 1993. Tetrazolium-based cell bioassay for neurotoxins active on voltage-sensitive sodium channels: semiautomated assay for saxitoxins, brevetoxins, and ciguatoxins. Anal. Biochem. 214: 190–194.
Norton S. 1996a. Toxic effects of plants. In: Klaassen C.D., Amdur M.O. and Doull J., Casarett & Doull's Toxicology. McGraw-Hill, New York, pp. 848.
Norton S. 1996b. Toxic effects of plants. In: Klaassen C.D., Amdur M.O. and Doull J., Casarett & Doull's Toxicology. McGraw-Hill, New York, pp. 842.
Norton S. 1996c. Toxic effects of plants. In: Klaassen C.D., Amdur M.O. and Doull J., Casarett & Doull's Toxicology. McGraw-Hill, New York, pp. 849.
Russel A.D. and Chopra I. 1990. Understanding Antibacterial Action and Resistance. Ellis Horwood Ltd., Publisher, Chichester, England.
Van Zorge J.A. 1996. Exposure to mixtures of chemical substances: is there a need for regulation? Food and Chem. Toxicol. 34: 1033–1036.
Yamashoji S., Nishimoto F., Usuda F., Kubota H. and Isshiki K. 1992. Application of the chemiluminescent assay to cytotoxicity test: detection of menadione-catalyzed H2O2production by viable cells. Anal. Biochem. 207: 255–260.
Yamashoji S. and Isshiki K. 1998. Cytotoxicity testing for evaluating food safety. Altern. Anim. Test Exp. 5: 146–161.
Yamashoji S. and Isshiki K. 1999. Novel cytotoxicity test based on menadione-catalyzed H2O2productivity for food safety evaluation. Cytotechnology 31: 45–51.
Yamazaki M., Nakajima T. and Fusetani N. 1995. Natutal Toxins. Kodansha, Tokyo.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yamashoji, S., Isshiki, K. Rapid detection of cytotoxicity of food additives and contaminants by a novel cytotoxicity test, menadione-catalyzed H2O2 production assay. Cytotechnology 37, 171–178 (2001). https://doi.org/10.1023/A:1020580818979
Issue Date:
DOI: https://doi.org/10.1023/A:1020580818979