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Use of the Direct Epifluorescent Filter Technique for the Enumeration of Viable and Total Acetic Acid Bacteria from Vinegar Fermentation

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Abstract

A rapid epifluorescence staining method using the LIVE/DEAD® BacLight™ Bacterial Viability kit was applied to differentiate both viable and total counts of acetic acid bacteria in vinegar fermentation. The results obtained were compared with those from other measurement techniques: 4′,6-diamidine-2phenyl indole (DAPI) and 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) and colony counts. BacLight total counts were comparable to DAPI (differing by <3.5%). BacLight viable counts were similar to CTC counts but considerable higher than colony-forming cells in plates.

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Authors and Affiliations

  1. Department of Chemical Engineering, Faculty of Sciences, University of Cádiz, Campus Rio San Pedro, 11510, Puerto Real, Cádiz, Spain

    M. M. Mesa, M. Macías & D. Cantero

  2. Laboratory of Bioenergetics and Microbiology, University of Geneva, Uni Bastions, 3 place I'

    F. Barja & F. Barja

Authors
  1. M. M. Mesa
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  2. M. Macías
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  3. D. Cantero
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  4. F. Barja
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Correspondence to M. M. Mesa.

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Mesa, M.M., Macías, M., Cantero, D. et al. Use of the Direct Epifluorescent Filter Technique for the Enumeration of Viable and Total Acetic Acid Bacteria from Vinegar Fermentation. Journal of Fluorescence 13, 261–265 (2003). https://doi.org/10.1023/A:1025094017265

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