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Fluorescence and amino acid characteristics of molecular size fractions of DOM in the waters of Lake Biwa

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Abstract

Dissolved organic matter (DOM) in the waters from Lake Biwa, Japan was fractionated using tangential flow ultrafiltration, and subsequently characterized by fluorescence properties and amino acids. While major dissolved organic carbon (DOC), UV absorbance (Abs), humic-like fluorescence (Flu) and total hydrolyzed amino acids (THAA) occurred in the less than 5 kDa molecular size fraction, they were not evenly distributed among various molecular size fractions. Flu/Abs ratios increased, and THAA/DOC ratios decreased with decreasing molecular size. Humic-like fluorescence occurred in all molecular size fractions, but protein-like fluorescence only occurred in the 0.1 μm-GF/F fraction. Subtle differences in amino acid compositions (both individuals and functional groups) were observed between various molecular size fractions, this may indicate the occurrence of DOM degradation from higher to lower molecular weight. The results reported here have significance for further understanding the sources and nature of DOM in aquatic environments.

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References

  • Berdie L., Grimalt J.O. and Gjessing E.T. 1995. Combined fatty acids and amino acids in the dissolved + colloidal and particulate fractions of the waters from a dystrophic lake. Org. Geochem. 23: 343–353.

    Google Scholar 

  • Buesseler K.O., Bauer J.E., Chen R.F., Eglington T.I., Gustafsson O., Landing W. et al. 1996. Sampling marine colloids using cross-flow filtration: Overview and results from an intercomparison study. Mar. Chem. 55: 1–31.

    Google Scholar 

  • Burdige D.J. and Martens C.S. 1988. Biogeochemical cycling in an organic-rich coastal marine basin. 10. The role of amino acids in sedimentary carbon and nitrogen cycling. Geochim. Cosmochim. Acta 52: 1571–1584.

    Google Scholar 

  • Carlson D., Brann M.L., Mague T.H. and Mayer L.M. 1985. Molecular size distribution of dissolved organic materials in seawater determined by ultrafiltration: a re-examination. Mar. Chem. 16: 155–171.

    Google Scholar 

  • Coble P.G., Green S.A., Blough N.V. and Gagosian R.B. 1990. Characterization of dissolved organic matter in the Black Sea by fluorescence spectroscopy. Nature 348: 432–435.

    Google Scholar 

  • Coble P.G. 1996. Characterization of marine and terrestrial DOM in seawater using excitation-emission matrix spectroscopy. Mar. Chem. 51: 325–346.

    Google Scholar 

  • Coffin R.B. 1989. Bacterial uptake of dissolved free and combined amino acids in estuarine waters. Limnol. Oceanogr. 34: 531–542.

    Google Scholar 

  • Colombo J.C., Silverberg N. and Gearing J.N. 1998. Amino acid biogeochemistry in the Laurentian Trough: vertical fluxes and individual reactivity during early diagenesis. Org. Geochem. 29: 933–945.

    Google Scholar 

  • Dauwe B. and Middelburg J.J. 1998. Amino acids and hexosamines as indictors of organic matter degradation state in North Sea sediments. Limnol. Oceanogr. 43: 783–798.

    Google Scholar 

  • Del Castillo C.E., Coble P.G., Morell J.M., Lopez J.M. and Corredor J.E. 1999. Analysis of the optical properties of the Orinoco River plume by adsorption and fluorescence spectroscopy. Mar. Chem. 66: 35–51.

    Google Scholar 

  • Determann S., Reuter R., Wagner P. and Willkomm R. 1994. Fluorescent matter in the eastern Atlantic Ocean. Part 1: method of measurement and near-surface distribution. Deep-Sea Res. 41: 659–675.

    Google Scholar 

  • Determann S., Lobbes J.M., Reuter R. and Rullkotter J. 1998. Ultraviolet fluorescence excitation and emission spectroscopy of marine algae and bacteria. Mar. Chem. 62: 137–156.

    Google Scholar 

  • Ewald M., Berger R. and Visser S.A. 1988. UV-visible absorption and fluorescence properties of fulvic acids of microbial origin as functions of their molecular sizes. Geoderma 43: 11–20.

    Google Scholar 

  • Gonzalez J.M., Grimalt J. and Albaiges J. 1983. Amino acid composition of sediments from a deltaic environment. Mar. Chem. 14: 61–71.

    Google Scholar 

  • Guo L., Coleman C.H. Jr and Santschi P.H. 1994. The distribution of colloidal and dissolved organic carbon in the Gulf of Mexico. Mar. Chem. 45: 105–119.

    Google Scholar 

  • Guo L., Santschi P.H. and Warnken K.W. 1995. Dynamics of dissolved organic carbon in oceanic environments. Limnol. Oceanogr. 40: 1392–1403.

    Google Scholar 

  • Guo L. and Santschi P.H. 1996. A critical evaluation of the cross-flow ultrafiltration techniques for sampling colloidal organic carbon in seawater. Mar. Chem. 55: 113–127.

    Google Scholar 

  • Guo L. and Santschi P.H. 1997. Isotopic and elemental characterization of colloidal organic matter from the Chesapeake Bay and Galveston Bay. Mar. Chem. 59: 1–15.

    Google Scholar 

  • Hart B.T., Sdraulig S. and Jones M.J. 1992. Behavior of copper and zinc added to the Tambo River, Australia, by a metal-enriched spring. Aust. J. Mar. Freshwater Res. 43: 457–489.

    Google Scholar 

  • Harvey H.R., Tuttle J.H. and Bell J.T. 1995. Kinetics of phytoplankton decay during simulated sedimentation: changes in biochemical composition and microbial activity under oxic and anoxic conditions. Geochem. Cosmochim. Acta 59: 3367–3377.

    Google Scholar 

  • Hollibaugh J.T. and Azam F. 1983. Microbial degradation of dissolved proteins in seawater. Limnol. Oceanogr. 28: 1104–1116.

    Google Scholar 

  • Lee C. and Cronin C. 1984. The vertical flux of particulate nitrogen in the sea: Decomposition of amino acids in the Peru upwelling area and the equatorial Atlantic. J. Mar. Res. 40: 227–251.

    Google Scholar 

  • Lindroth P. and Mopper K. 1979. High performance liquid chromatographic determination of sub-picomolar amounts of amino acids by precolumn fluorescence derivatization with o-phthaldialdehyde. Anal. Chem. 51: 1667–1674.

    Google Scholar 

  • Maita Y., Montani S. and Ishii J. 1982. Early diagenesis of amino acids in Okhotsk Sea sediments. Deep-sea Res. 29: 485–498.

    Google Scholar 

  • Martin J.M., Dai M.H. and Cauwet G. 1995. Significance of colloids in the biogeochemical cycling of organic carbon and trace metals in the Venice Lagoon. Limnol. Oceanogr. 40: 119–131.

    Google Scholar 

  • Mayer L.M., Schick L.L. and Loder T.C. III 1999. Dissolved protein fluorescence in two Maine estuaries. Mar. Chem. 64: 171–179.

    Google Scholar 

  • Mayers P.A., Leenheer M.J., Eadie B.J. and Maule S.J. 1984. Organic geochemistry of suspended and setting particulate matter in Lake Michigan. Geochim. Cosmochim. Acta 48: 443–452.

    Google Scholar 

  • Miyajima T., Yamada Y., Wada E., Nakajima T., Koitabashi T., Hanba Y.T. et al. 1997. Distribution of greenhouse gases, nitrite, and (?13C of dissolved inorganic carbon in Lake Biwa: Implications for hydrolimnetic metabolism. Biogeochem 36: 205–221.

    Google Scholar 

  • Mopper K. and Schultz C.A. 1993. Fluorescence as a possible tool for studying the nature and water column distribution of DOC components. Mar. Chem. 41: 229–238.

    Google Scholar 

  • Mopper K., Feng Z.M., Bentjen S.B. and Chen R.F. 1996. Effects of cross-flow filtration on the absorption and fluorescence properties of seawater. Mar. Chem. 55: 53–74.

    Google Scholar 

  • Moran M.A., Sheldon W.M. Jr and Zepp R.G. 2000. Carbon loss and optical property changes during long-term photochemical and biological degradation of estuarine dissolved organic matter. Limnol. Oceanogr. 45: 1254–1264.

    Google Scholar 

  • Orlandini K.A., Penrose W.R., Harvey B.R., Lovett M.B. and Findlay M.W. 1990. Colloidal behavior of Actinides in an oligotropic lake. Environ. Sci. Technol. 24: 706–712.

    Google Scholar 

  • Robertson K.J., Williams P.M. and Bada J.L. 1987. Acid hydrolysis of dissolved combined amino acids in seawater: A precautionary note. Limnol. Oceanogr. 32: 996–997.

    Google Scholar 

  • Salbu B., Bjornstad H.E., Lydersen E. and Pappas A.C. 1987. Size fractionation techniques combined with INAA for speciation purpose. J. Radioanal. Nucl. Chem. 5: 169–184.

    Google Scholar 

  • Senesi N. 1990. Molecular and quantitative aspects of the chemistry of fulvic acid and its interactions with metal ions and organic chemicals: Part II. The fluorescence spectroscopy approach. Anal. Chem. Acta 232: 77–106.

    Google Scholar 

  • Siezen R.J. and Mague T.H. 1978. Amino acids in suspended particulate matter from oceanic and coastal waters of the Pacific. Mar. Chem. 6: 215–231.

    Google Scholar 

  • Smith R.G. Jr 1976. Evaluation of combined applications of ultrafiltration and complexation capacity techniques to natural waters. Anal. Chem. 48: 74–76.

    Google Scholar 

  • Steinberg S.M., Venkatesan M.I. and Kaplan R. 1987. Organic geochemistry of sediments from the continental margin off south New England, USA. I. Amino acids, carbohydrates and lignin. Mar. Chem. 21: 249–265.

    Google Scholar 

  • Stewart A.J. and Wetzel R.G. 1980. Fluorescence:absorbance ratios: a molecular-weight tracer of dissolved organic matter. Limnol. Oceanogr. 25: 559–564.

    Google Scholar 

  • Traganza E.D. 1969. Fluorescence excitation and emission spectra of dissolved organic matter in seawater. Bull. Mar. Sci. 19: 897–904.

    Google Scholar 

  • Wolfbeis O.S. 1985. The fluorescence of organic natural products. In: Schulman S.G. (ed.), Molecular Luminescence Spectroscopy, Methods and Applications, Part I. Vol. 77. Wiley Interscience, New York, pp. 167–370.

    Google Scholar 

  • Wu F.C. and Tanoue E. 2001a. Molecular mass distribution and fluorescence characteristics of dissolved organic ligands for copper(II) in Lake Biwa, Japan. Org. Geochem. 32: 11–20.

    Google Scholar 

  • Wu F.C. and Tanoue E. 2001b. Sensitive determination of dissolved tryptophan in freshwater by alkaline hydrolysis and HPLC. Anal. Sci. 17: 1063–1067.

    Google Scholar 

  • Wu F.C., Midorikawa T. and Tanoue E. 2001. Fluorescence properties of organic ligands for copper(II) in Lake Biwa and its rivers. Geochem. J. 35: 333–346.

    Google Scholar 

  • Zepp R.G. 1998. Environmental photoprocesses involving natural organic matter. In: Frimmel F.H. and Christman R.F. (eds), Humic Substances and their Role in the Environment. Wiley, pp. 193–214.

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  1. F.C. Wu
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  2. E. Tanoue
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  3. C.Q. Liu
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Wu, F., Tanoue, E. & Liu, C. Fluorescence and amino acid characteristics of molecular size fractions of DOM in the waters of Lake Biwa. Biogeochemistry 65, 245–257 (2003). https://doi.org/10.1023/A:1026074318377

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