Abstract
We studied the capacity of colloidal gold for enhancing specific and nonspecific immune response in laboratory animals (rabbits, rats, and mice) immunized with antigens of various nature. The antibody titers obtained with colloidal gold as a carrier were higher as compared to the standard immunization techniques (free antigen or its combination with Freund's adjuvant). Application of colloidal gold also enhanced nonspecific immune responses, such as lysozyme concentration in the blood, activity of the complement system proteins, as well as phagocytic and bactericidal activities. The antibodies were tested by immunodot assay using gold markers. Immunization of the animals with colloidal gold conjugates with haptens or complete antigens (without other adjuvants) was shown to induce the production of highly active antibodies. In addition, the amount of antigen used for animal immunization with colloidal gold was an order of magnitude lower, compared to immunization with complete Freund's adjuvant. This fact can be evidence for adjuvant properties of colloidal gold proper.
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REFERENCES
Abraham, G.E. and Himmel, P.B., Management of Rheumatoid Arthritis: Rationale for the Use of Colloidal Metallic Gold, J. Nutr. Med. 1997, vol. 7, pp. 295-305.
Andreev, S.M., Babakhin, A.A., Petrukhina, A.O., Romanova, V.S., Parnes, Z.N., and Petrov, R.V., Immunogenic and Allergenic Properties of Fulleren Conjugates with Amino Acids and Proteins, Dokl. Ross. Akad. Nauk, 2000, vol. 370, pp. 261-264.
Antibodies —A Practical Approach, Catty, D., Ed., Washington: IRL, 1989. Translated under the title Antitela, Moscow: Mir, 1991.
Chen, J., Zou, F., Wang, N., Xie, S., and Zhang, X., Production and Application of LPA Polyclonal Antibody, Bioorg. Med. Chem. Lett., 2000, vol. 10, pp. 1691-1693.
Colloidal Gold: Principles, Methods and Applications, Hayat, M.A., Ed., San Diego: Academic, 1989, vol. 1.
Dou, X., Jung, Y.M., Yamamoto, H., Doi, S., and Ozaki, Y., Near-Infrared Excited Surface-Enhanced Raman Scattering of Biological Molecules on Gold Colloid I: Effects of pH of the Solutions of Amino Acids and of Their Polymerization, Appl. Spectrosc., 1999, vol. 53, pp. 133-138.
Dykman, L.A. and Bogatyrev, V.A., Colloidal Gold in Solid-Phase Assays: A Review, Biochemistry (Moscow), 1997, vol. 62, pp. 411-418.
Dykman, L.A., Matora, L.Yu., and Bogatyrev, V.A., Use of Colloidal Gold to Obtain Antibiotin Antibodies, J. Microbiol. Meth., 1996, vol. 24, pp. 247-248.
Frens, G., Controlled Nucleation for the Regulation of the Particle Size in Monodisperse Gold Suspensions, Nature Phys. Sci., 1973, vol. 241, pp. 20-22.
Fukasawa, M., Liposome Oligomannan-Coated with Neoglycolipid, a New Candidate for a Safe Adjuvant for Induction of CD8+ Cytotoxic T-Lymphocytes, FEBS Lett., 1998, vol. 441, pp. 353-356.
Immunologische Arbeitsmethoden, Friemel, H., Ed., Jena: Fischer, 1991. Translated under the title Immunologicheskie metody, Moscow: Meditsina, 1987.
Kovalev, I.E. and Polevaya, O.Yu., Biokhimicheskie osnovy immuniteta k nizkomolekulyarnym khimicheskim soedineniyam (Biochemical Bases of Immunity against Low Molecular Weight Chemicals), Moscow: Nauka, 1985.
Kreuter, J., Nanoparticles as Adjuvants for Vaccines, Pharm. Biotechnol., 1995, vol. 6, pp. 463-472.
McCafferty, J., Griffiths, A.D., Winter, G., and Chiswell, D.J., Phage Antibodies: Filamentous Phage Displaying Antibody Variable Domains, Nature, 1990, vol. 348, pp. 552-554.
McEwen, J., Levi, R., Horwitz, R. J., and Arnon, R., Synthetic Recombinant Vaccine Expressing Influenza Haemagglutinin Epitope in Salmonella Flagellin Leads to Partial Protection in Mice, Vaccine, 1992, vol. 10, pp. 405-411.
Muller, S., Peptide-Carrier Conjugation, Synthetic Peptides as Antigens, Van Regenmartel, M.H.V. and Muller, S., Eds., Amsterdam: Elsevier, 1999, pp. 79-131.
Nesmeyanov, V.A., Golovina, T.V., Valyakina, T.I., Andronova, T.M., and Ivanov, V.T., Cellular and Molecular Mechanisms of Biological Activity of Muramyl Peptides, in Immunotherapy of Infections, Masihi, N., Ed., New York: Marcel Dekker, 1994, pp. 213-223.
Paster, E.U., Ovod, V.V., Pozur, V.K., and Vikhot', N.E., Immunologiya: Praktikum (Immunology: A Practical Course), Kiev: Vishcha Shkola, 1989.
Petrov, R.V. and Khaitov, R.M., Vaccines of a New Generation Based on Synthetic Polyons: History of Design, Phenomenology and Mechanisms of Action, and Introduction into Practice, Immunologiya, 1998, no. 1, pp. 4-11.
Pow, D.V. and Crook, D.K., Extremely High Titre Polyclonal Antisera against Small Neurotransmitter Molecules: Rapid Production, Characterization and Use in Light and Electron-Microscopic Immunocytochemistry, J. Neurosci. Meth., 1993, vol. 48, pp. 51-63.
Shiosaka, S., Kiyama, H., Wanaka, A., and Tohyama, M., A New Method for Producing a Specific and High Titre Antibody against Glutamate Using Colloidal Gold as a Carrier, Brain Res., 1986, vol. 382, pp. 399-403.
Tomii, A. and Masugi, F., Production of Anti-Platelet-Activating Factor Antibodies by the Use of Colloidal Gold as Carrier, Jpn. J. Med. Sci. Biol., 1991, vol. 44, pp. 75-80.
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Dykman, L.A., Sumaroka, M., Staroverov, S.A. et al. Immunogenic Properties of Colloidal Gold. Biology Bulletin 31, 75–79 (2004). https://doi.org/10.1023/B:BIBU.0000014358.98422.9c
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DOI: https://doi.org/10.1023/B:BIBU.0000014358.98422.9c