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Chitosan sponges as tissue engineering scaffolds for bone formation

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Abstract

Rat calvarial osteoblasts were grown in porous chitosan sponges fabricated by freeze drying. The prepared chitosan sponges had a porous structure with a 100–200 μm pore diameter, which allowed cell proliferation. Cell density, alkaline phosphatase activity and calcium deposition were monitored for up to 56 d culture. Cell numbers were 4 × 106 (day 1), 11 × 106 (day 28) and 12 × 106 (day 56) per g sponge. Calcium depositions were 9 (day 1), 40 (day 28) and 48 (day 56) μg per sponge. Histological results corroborated that bone formation within the sponges had occurred. These results show that chitosan sponges can be used as effective scaffolding materials for tissue engineered bone formation in vitro.

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Authors and Affiliations

  1. Department of Periodontology, College of Dentistry, Seoul National University, 28-2 Yongon-Dong, Chongno-Ku, Seoul, 110-749, South Korea

    Yang-Jo Seol, Yong-Moo Lee, Young -Ku, In-Chul Rhyu, Soo-Boo Han & Chong-Pyoung Chung

  2. Department of Periodontology, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Ilwon-dong, Kangnam-Ku, Seoul, 135-710, South Korea

    Yang-Jo Seol

  3. College of Pharmacy, Ewha Womans University, 11-1 Daehyun-dong, Seodaemun-Ku, Seoul, 120-750, South Korea

    Jue-Yeon Lee & Seung-Jin Lee

  4. Department of Craniomaxillofacial Reconstructive Science, College of Dentistry, Seoul National University, 28-2 Yongon-Dong, Chongno-Ku, Seoul, 110-749, South Korea

    Yoon-Jeong Park & In-Chul Rhyu

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  1. Yang-Jo Seol
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  2. Jue-Yeon Lee
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  4. Yong-Moo Lee
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  5. Young -Ku
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  6. In-Chul Rhyu
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  7. Seung-Jin Lee
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Seol, YJ., Lee, JY., Park, YJ. et al. Chitosan sponges as tissue engineering scaffolds for bone formation. Biotechnology Letters 26, 1037–1041 (2004). https://doi.org/10.1023/B:BILE.0000032962.79531.fd

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  • DOI: https://doi.org/10.1023/B:BILE.0000032962.79531.fd

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