Abstract
The use of Pichia pastoris for protein production was simplified by creation of fusion proteins containing green fluorescent protein (GFP) and the product of interest. Human interleukin-2 (hIL-2) was used as a model product: GFP enabled clear identification of fusion protein expression and, more importantly, the quantification of hIL-2. Although GFP fusions for bioprocess monitoring have been demonstrated in other hosts, this is its first use in P. pastoris.
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Cha, H.J., Dalal, N.G. & Bentley, W.E. In vivo monitoring of intracellular expression of human interleukin-2 using green fluorescent protein fusion partner in Pichia pastoris . Biotechnology Letters 26, 1157–1162 (2004). https://doi.org/10.1023/B:BILE.0000035489.09417.d4
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DOI: https://doi.org/10.1023/B:BILE.0000035489.09417.d4