Growth of Cell-strains and Primary Cells on Micro-carriers in Homogeneous Culture

Abstract

THERE are obvious advantages in culturing tissue cells in suspension instead of in monolayers and in using the suspension as a substrate for virus multiplication. Culture conditions for cell growth and virus multiplication can be studied and large scale production can be achieved more easily. The growth of cells from cell-lines, in suspension culture, as separate units or small clumps, has already been demonstrated. In contrast, nobody has yet succeeded in growing diploid and primary cells by this method^1,2, for the cells have become aneuploid or did not grow at all in suspension culture³.