Abstract
The glutamate dehydrogenase gene of Escherichia coli has been cloned into broad host-range plasmids and can complement glutamate synthase mutants of Methylophilus methylotrophus. Assimilation of ammonia via glutamate dehydrogenase is more energy-efficient than via glutamate synthase, thus the recombinant organism converts more growth substrate, methanol, into cellular carbon.
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Windass, J., Worsey, M., Pioli, E. et al. Improved conversion of methanol to single-cell protein by Methylophilus methylotrophus. Nature 287, 396–401 (1980). https://doi.org/10.1038/287396a0
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DOI: https://doi.org/10.1038/287396a0
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