Abstract
The construction of stable blood vessels is a fundamental challenge for tissue engineering in regenerative medicine. Although certain genes can be introduced into vascular cells to enhance their survival and proliferation, these manipulations may be oncogenic. We show here that a network of long-lasting blood vessels can be formed in mice by co-implantation of vascular endothelial cells and mesenchymal precursor cells, by-passing the need for risky genetic manipulations. These networks are stable and functional for one year in vivo.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Orlidge, A. & D'Amore, P. A. J. Cell Biol. 105, 1455–1462 (1987).
Hirschi, K. K., Rohovsky, S. A. & D'Amore, P. A. J. Cell Biol. 141, 805–814 (1998).
Darland, D. C. & D'Amore, P. A. Angiogenesis 4, 11–20 (2001).
Jain, R. K., Munn, L. L. & Fukumura, D. Nature Rev. Cancer 2, 266–276 (2002).
Schechner, J. S. et al. Proc. Natl Acad. Sci. USA 97, 9191–9196 (2000).
Jain, R. K. Nature Med. 9, 685–693 (2003).
Yang, J. et al. Nature Biotechnol. 19, 219–224 (2001).
McKee, J. A. et al. EMBO Rep. 4, 633–638 (2003).
Yamashita, J. et al. Nature 408, 92–96 (2000).
Simper, D., Stalboerger, P. G., Panetta, C. J., Wang, S. & Caplice, N. M. Circulation 106, 1199–1204 (2002).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Supplementary information
Supplementary Information
Includes information on: preparation of the 3-D construct for tissue engineered blood vessels; vascular permeability measurement; and arteriolar contractility assay. (DOC 31 kb)
Supplementary Movie
3-D presentation of tissue engineered blood vessels. HUVECs and 10T1/2 cells were seeded in the 3-D constructs and implanted in the animals. EGFP-expressing HUVECs (green) and perfused blood vessels (red) were visualized by multi-photon laser-scanning microscopy (Ref. 4). At day 28, blood perfusion could be seen in all layers of the construct. Thickness of the construct is 165 µm. Image is 270 µm across. (MOV 1006 kb)
Rights and permissions
About this article
Cite this article
Koike, N., Fukumura, D., Gralla, O. et al. Creation of long-lasting blood vessels. Nature 428, 138–139 (2004). https://doi.org/10.1038/428138a
Issue Date:
DOI: https://doi.org/10.1038/428138a
This article is cited by
-
A critical review of fibrous polyurethane-based vascular tissue engineering scaffolds
Journal of Biological Engineering (2022)
-
Engineering the multiscale complexity of vascular networks
Nature Reviews Materials (2022)
-
Isolation of ready-made rat microvessels and its applications in effective in vivo vascularization and in angiogenic studies in vitro
Nature Protocols (2022)
-
Challenges and Perspectives on the Use of Pericytes in Tissue Engineering
Current Tissue Microenvironment Reports (2022)
-
Contrast agents for photoacoustic imaging: a review of stem cell tracking
Stem Cell Research & Therapy (2021)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.