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Intrinsic bias and public rearrangements in the human immunoglobulin Vλ light chain repertoire

Genes & Immunity volume 14, pages 271–276 (2013)Cite this article

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Abstract

The immunoglobulin lambda (IGL) repertoires from two unrelated human blood samples, three NOD-scid-IL2Rγnull mice engrafted with human hematopoietic stem cells and two pairs of monozygotic twin blood samples were determined by Roche 454 sequencing to generate a total of about 700 000 IGL sequences. We applied bioinformatic analysis to examine IGL repertoires wherein, surprisingly, ⩾20% of CDR-L3 peptide sequences were ‘public’ (shared across individuals); moreover, full-length IGL protein sequences (VJ recombinants) were also present in the public domain. Subtle yet significant differences in CDR-L3 nontemplated nucleotide addition, IGL V-gene family usage, and amino-acid composition distinguished the public CDR-L3 groups from the private groups. These data suggest that public CDR-L3 intervals can arise by intrinsic genetic mechanisms irrespective of different B-cell developmental milieu (human versus humanized mouse). Furthermore, the occurrence of identical public IGL protein sequences indirectly suggest the positive selection (evolutionary, somatic or both) of particular IGL chains independent of the immunoglobulin heavy chain.

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Acknowledgements

We acknowledge Jaime O’Neal and Chhaya Das for their technical assistance. We also acknowledge Arvind Rajpal, Tracy Kuo and Marina Sirota for providing the Rinat–Pfizer twin sequences. We are grateful to George Georgiou for his insightful commentary and general support and collegiality.

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Authors and Affiliations

  1. Department of Biomedical Engineering, University of Texas at Austin, Austin, TX, USA

    K H Hoi

  2. Section of Molecular Genetics and Microbiology, University of Texas at Austin, Austin, TX, USA

    G C Ippolito

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  1. K H Hoi
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Correspondence to G C Ippolito.

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Hoi, K., Ippolito, G. Intrinsic bias and public rearrangements in the human immunoglobulin Vλ light chain repertoire. Genes Immun 14, 271–276 (2013). https://doi.org/10.1038/gene.2013.10

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