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Preparation and hybridization analysis of DNA/RNA from E. coli on microfabricated bioelectronic chips

Nature Biotechnology volume 16, pages 541–546 (1998)Cite this article

Abstract

Escherichia coli were separated from a mixture containing human blood cells by means of dielec-trophoresis and then subjected to electronic lysis followed by proteolytic digestion on a single microfabricated bioelectronic chip. An alternating current electric field was used to direct the bacteria to 25 microlocations above individually addressable platinum microelectrodes. The platinum electrodes were 80 μm in diameter and had center-to-center spacings of 200 μm. After the isolation, the bacteria were lysed by a series of high-voltage pulses. The lysate contained a spectrum of nucleic acids including RNA, plasmid DNA, and genomic DNA. The lysate was further examined by electronically enhanced hybridization on separate bioelectronic chips. Dielectrophoretic separation of cells followed by electronic lysis and digestion on an electronically active chip may have potential as a sample preparation process for chip-based hybridization assays in an integrated DNA/RNA analysis system.

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Authors and Affiliations

  1. Nanogen, Inc., 10398 Pacific Center Ct., San Diego, CA, 92121

    Jing Cheng, Edward L. Sheldon, Lei Wu, Adam Uribe, Louis O. Gerrue, John Carrino, Michael J. Heller & James P. O'Connell

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  1. Jing Cheng
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  2. Edward L. Sheldon
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  4. Adam Uribe
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  7. Michael J. Heller
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  8. James P. O'Connell
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Correspondence to Jing Cheng.

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Cheng, J., Sheldon, E., Wu, L. et al. Preparation and hybridization analysis of DNA/RNA from E. coli on microfabricated bioelectronic chips. Nat Biotechnol 16, 541–546 (1998). https://doi.org/10.1038/nbt0698-541

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