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Imaging without lenses: achievements and remaining challenges of wide-field on-chip microscopy

Nature Methods volume 9, pages 889–895 (2012)Cite this article

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Abstract

We discuss unique features of lens-free computational imaging tools and report some of their emerging results for wide-field on-chip microscopy, such as the achievement of a numerical aperture (NA) of ∼0.8–0.9 across a field of view (FOV) of more than 20 mm2 or an NA of ∼0.1 across a FOV of ∼18 cm2, which corresponds to an image with more than 1.5 gigapixels. We also discuss the current challenges that these computational on-chip microscopes face, shedding light on their future directions and applications.

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Figure 1: Partially coherent lens-free on-chip microscope.
Figure 2: Lens-free gigapixel imaging using a CCD sensor chip.
Figure 3: High–numerical aperture lens-free imaging using a color CMOS sensor chip.

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Acknowledgements

A.O. gratefully acknowledges the support of the Presidential Early Career Award for Scientists and Engineers, Army Research Office Young Investigator Award, National Science Foundation CAREER Award, Office of Naval Research Young Investigator Award and National Institutes of Health Director′s New Innovator Award DP2OD006427 from the Office of the Director, National Institutes of Health.

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Authors and Affiliations

  1. Electrical Engineering Department, University of California, Los Angeles (UCLA), Los Angeles, California, USA

    Alon Greenbaum, Wei Luo, Ting-Wei Su, Zoltán Göröcs, Liang Xue, Serhan O Isikman, Ahmet F Coskun, Onur Mudanyali & Aydogan Ozcan

  2. Department of Bioengineering, UCLA, Los Angeles, California, USA

    Alon Greenbaum, Wei Luo, Ting-Wei Su, Zoltán Göröcs, Liang Xue, Serhan O Isikman, Ahmet F Coskun, Onur Mudanyali & Aydogan Ozcan

  3. Department of Information Physics and Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu, China

    Liang Xue

  4. California NanoSystems Institute, UCLA, Los Angeles, California, USA

    Aydogan Ozcan

  5. Department of Surgery, David Geffen School of Medicine, UCLA, Los Angeles, California, USA

    Aydogan Ozcan

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  1. Alon Greenbaum
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Correspondence to Aydogan Ozcan.

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A.O. is the founder of a start-up company that aims to commercialize optical imaging–based diagnostics tools.

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Greenbaum, A., Luo, W., Su, TW. et al. Imaging without lenses: achievements and remaining challenges of wide-field on-chip microscopy. Nat Methods 9, 889–895 (2012). https://doi.org/10.1038/nmeth.2114

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