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Photophysics and photochemistry of zinc phthalocyanine/bovine serum albumin adducts

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Abstract

Zinc phthalocyanine (ZnPc) is a well known Type II (singlet oxygen mediated) hydrophobic photosensitizer with potential use in PDT. We have found that the presence of bovine serum albumin diminishes the aggregation degree of ZnPc in aqueous solution, indicating that albumins could be potentially useful carriers for this type of photosensitizer in PDT. In order to explore the photochemical and photophysical behavior of ZnPc associated to the protein, we have evaluated triplet excited state lifetime and yield, dye bleaching, oxygen consumption, formation of carbonyls and peroxides, and the spontaneous chemiluminiscence emitted after photolysis. The results show that dye association to BSA modifies the photophysics and photochemistry of ZnPC. In particular the decreased yield of long lived triplets suggests singlet state and/or static triplet quenching of the bound dye by the hostprotein.

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Authors and Affiliations

  1. Facultad de Química, Departamento de Química Física, Pontificia Universidad Católica de Chile, Avda. Vicuña Mackenna 4860, Casilla 306, (536), Santiago, Chile

    Emilio Alarcón, Ana Maria Edwards, Angélica M. Garcia & Marcelo Muñoz

  2. Departamento de Ciencias del Ambiente, Facultad de Química y Biología, Universidad de Santiago de Chile, Avenida Libertador Bernardo O’Higgins 3363, Casilla 40, Correo, 33, Santiago, Chile

    Alexis Aspée & Eduardo A. Lissi

  3. Instituto de Química del NOA (INQUINOA-CONICET), Facultad de Agronomia y Agroindustrias, Universidad Nacional de Santiago del Estero, Av. Belgrano (S) 1912, Santiago del Estero, Argentina

    Claudio D. Borsarelli

Authors
  1. Emilio Alarcón
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  2. Ana Maria Edwards
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  3. Angélica M. Garcia
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  4. Marcelo Muñoz
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  5. Alexis Aspée
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  6. Claudio D. Borsarelli
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  7. Eduardo A. Lissi
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Correspondence to Ana Maria Edwards.

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Alarcón, E., Edwards, A.M., Garcia, A.M. et al. Photophysics and photochemistry of zinc phthalocyanine/bovine serum albumin adducts. Photochem Photobiol Sci 8, 255–263 (2009). https://doi.org/10.1039/b815726j

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