Improved bone formation and ingrowth for additively manufactured porous Ti6Al4V bone implants with strontium laden nanotube array coating

Tian-Xiao Han; Bei Chang; Xin Ding; Guang-Na Yue; Wen Song; Hui-Ping Tang; Liang Jia; Ling-Zhou Zhao; Yu-Mei Zhang

doi:10.1039/C5RA20370H

Improved bone formation and ingrowth for additively manufactured porous Ti₆Al₄V bone implants with strontium laden nanotube array coating†

Tian-Xiao Han,*^a Bei Chang,*^a Xin Ding,^a Guang-Na Yue,^a Wen Song,^a Hui-Ping Tang,^c Liang Jia,^c Ling-Zhou Zhao*^b and Yu-Mei Zhang*^a

Author affiliations

* Corresponding authors

^a State Key Laboratory of Military Stomatology, Department of Prosthetic Dentistry, School of Stomatology, The Fourth Military Medical University, No. 145 West Changle Road, Xi’an 710032, China
E-mail: wqtzym@fmmu.edu.cn
Fax: +86 29 84776096
Tel: +86 29 84776090

^b State Key Laboratory of Military Stomatology, Department of Periodontology, School of Stomatology, The Fourth Military Medical University, No. 145 West Changle Road, Xi’an 710032, China
E-mail: zhaolingzhou1983@hotmail.com

^c State Key Laboratory of Porous Metal Materials, Northwest Institute for Nonferrous Metal Research, Xi’an 710016, China

Abstract

A Ti implant with an interconnected porous structure may be a better choice for bone defect restoration; an important issue is to improve its bone formation and ingrowth abilities. In this study, a porous Ti₆Al₄V implant is fabricated via electron beam melting (EBM) technology with a precisely controlled pore shape and size as well as good interconnectivity. Anodization treatment and further Sr incorporation give rise to an even distribution of the titania nanotube array (NT) and a strontium-laden NT (NTSr) coating on the outer and inner surface of the porous implant, which significantly enhance its hydrophilicity. The NT and NTSr coatings, especially NTSr, significantly improve the in vitro infiltration and osteogenic differentiation of bone marrow mesenchymal stem cells (BMMSCs) as well as the in vivo bone formation and ingrowth abilities of the porous implant. The data also show that the pore size differentially influences the biological effect of the porous implant, especially after surface modification. The smaller pores (600 μm) favor in vitro BMMSC proliferation and osteogenic differentiation and in vivo new bone mass formation, while the larger pores (800 μm) favor in vitro cell ingrowth and in vivo bone ingrowth. Our study suggests that the NTSr coating is very promising for porous implant applications to improve their biological performance and also uncover the differential effect of the pore size on the biological effect of the porous implant.

Article information

https://doi.org/10.1039/C5RA20370H

Article type

Paper

Submitted

01 Oct 2015

Accepted

18 Jan 2016

First published

22 Jan 2016

Download Citation

RSC Adv., 2016,6, 13686-13697

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Improved bone formation and ingrowth for additively manufactured porous Ti₆Al₄V bone implants with strontium laden nanotube array coating

T. Han, B. Chang, X. Ding, G. Yue, W. Song, H. Tang, L. Jia, L. Zhao and Y. Zhang, RSC Adv., 2016, 6, 13686 DOI: 10.1039/C5RA20370H

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