Issue 35, 2009
From the journal:

Journal of Materials Chemistry

Glycosaminoglycan-functionalized gold nanorods: interactions with cardiac cells and type I collagen†‡

Christopher G. Wilson,§a   Patrick N. Sisco,§b   Edie C. Goldsmith*a  and  Catherine J. Murphy*b  

* Corresponding authors

a University of South Carolina School of Medicine, Department of Cell Biology & Anatomy, Columbia, SC, USA
E-mail: edie.goldsmith@uscmed.sc.edu
Fax: +1-803-733-3153
Tel: +1-803-733-3129

b University of South Carolina, Department of Chemistry & Biochemistry, Columbia, SC, USA
E-mail: murphy@mail.chem.sc.edu
Fax: +1-803-777-9521
Tel: +1-803-777-3628

Abstract

The sulfated glycosaminoglycans (sGAG) heparin and chondroitin sulfate (CS) were immobilized on the surfaces of gold nanorods as part of a polyelectrolyte multilayer. The effects of these nanomaterials on the self-assembly of type I collagen were examined by turbidity assays and microscopy, and desorption of sGAG from nanomaterial-collagen composites was quantified biochemically. The interactions of sGAG-coated nanorods with cardiac cells were also explored through a collagen gel contraction assay and confocal microscopy. In contrast to soluble forms of sGAG, sGAG-coated nanorods consistently accelerated collagen fibrillogenesis. Soluble heparin, and heparin- and CS-coated nanorods inhibited cell-mediated contraction of collagen gels, whereas soluble CS did not. Both heparin and CS-coated nanorods were detected in the peri- and/or intra-cellular compartments of the cells, but there was no evidence of cytotoxicity over 72 h of culture. These results indicate that biological polyanions, such as sGAG, may be useful in the modification of nanoparticle surface chemistry for biological and/or therapeutic applications.

Graphical abstract: Glycosaminoglycan-functionalized gold nanorods: interactions with cardiac cells and type I collagen

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Article information

DOI
https://doi.org/10.1039/B902760B
Article type
Paper
Submitted
10 Feb 2009
Accepted
23 Apr 2009
First published
27 May 2009

J. Mater. Chem., 2009,19, 6332-6340

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Glycosaminoglycan-functionalized gold nanorods: interactions with cardiac cells and type I collagen

C. G. Wilson, P. N. Sisco, E. C. Goldsmith and C. J. Murphy, J. Mater. Chem., 2009, 19, 6332 DOI: 10.1039/B902760B

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