Herein, we describe a facile and feasible synthesis method for patterning nanogold particles onto magnetic mesoporous CoFe₂O₄ nanostructures (Au-MMNs) by using poly(vinyl pyrrolidone) (PVP) as cross-linker. Initially, mesoporous CoFe₂O₄ nanoparticles were initially synthesized with a thermal decomposition method by using mesoporous silica nanoparticles as templates, and then nanometre-sized gold particles were produced through the in situ reduction of the Au^III on the PVP-functionalized CoFe₂O₄. The as-prepared Au-MMNs were characterized by transmission electron microscopy (TEM), N₂ adsorption-desorption isotherms, UV-visible adsorption spectrometer, vibrating sample magnetometer (VSM) and X-ray photoelectron spectroscopy (XPS). Furthermore, we also demonstrate the conjugation capacity of the synthesized Au-MMNs toward biomolecules by using quartz crystal microbalance (QCM), and the possible application in the electrochemical immunoassays. Experimental results indicated that the resulting Au-MMNs display good conjugation capability toward the biomolecules, and excellent analytical properties for determination of target molecules.