Issue 29, 2014
From the journal:

RSC Advances

Exact tailoring of an ATP controlled streptavidin binding aptamer†

Tao Bing,‡a   Hongcheng Mei,‡ab   Nan Zhang,a   Cui Qi,a   Xiangjun Liua  and  Dihua Shangguan*a  

* Corresponding authors

a Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China
E-mail: sgdh@iccas.ac.cn
Fax: +86-10-62528509
Tel: +86-10-62528509

b Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China

Abstract

By replacing a stem of a streptavidin binding aptamer with a split ATP binding aptamer, we tailored a fused aptamer. Its binding ability to streptavidin could be controlled through the allosteric change induced by ATP binding. This aptamer engineering strategy could be used to construct other aptamer switches with desired functions.

Graphical abstract: Exact tailoring of an ATP controlled streptavidin binding aptamer

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Article information

DOI
https://doi.org/10.1039/C4RA00714J
Article type
Communication
Submitted
24 Jan 2014
Accepted
12 Mar 2014
First published
12 Mar 2014

RSC Adv., 2014,4, 15111-15114

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Exact tailoring of an ATP controlled streptavidin binding aptamer

T. Bing, H. Mei, N. Zhang, C. Qi, X. Liu and D. Shangguan, RSC Adv., 2014, 4, 15111 DOI: 10.1039/C4RA00714J

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