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Time-resolved methods in biophysics. 3. Fluorescence lifetime correlation spectroscopy

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Abstract

We present a thorough introduction into the recently developed fluorescence lifetime correlation spectroscopy (FLCS). The theoretical basis of FLCS is explained, and the method is applied to the study of a dynamic transition between two fluorescence lifetime states in a dye—protein complex.

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Authors and Affiliations

  1. Institute for Biological Information Processing 1, Forschungszentrum Jülich, D-52425, Jülich, Germany

    Ingo Gregor & Jörg Enderlein

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  1. Ingo Gregor
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  2. Jörg Enderlein
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Correspondence to Ingo Gregor.

Additional information

† Edited by T. Gensch and C. Viappiani. This paper is derived from the lecture given at the X School of Pure and Applied Biophysics “Time-resolved spectroscopic methods in biophysics” (organized by the Italian Society of Pure and Applied Biophysics), held in Venice in January 2006.

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Gregor, I., Enderlein, J. Time-resolved methods in biophysics. 3. Fluorescence lifetime correlation spectroscopy. Photochem Photobiol Sci 6, 13–18 (2007). https://doi.org/10.1039/b610310c

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