Bioinspired preparation of polydopamine microcapsule for multienzyme system construction

Lei Zhang; Jiafu Shi; Zhongyi Jiang; Yanjun Jiang; Shizhang Qiao; Jian Li; Rui Wang; Ruijie Meng; Yuanyuan Zhu; Yang Zheng

doi:10.1039/C0GC00432D

Bioinspired preparation of polydopamine microcapsule for multienzyme system construction†

Lei Zhang,^ab Jiafu Shi,^ab Zhongyi Jiang,*^ab Yanjun Jiang,^c Shizhang Qiao,^d Jian Li,^a Rui Wang,^a Ruijie Meng,^a Yuanyuan Zhu^a and Yang Zheng^a

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* Corresponding authors

^a Key Laboratory for Green Technology of Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China
E-mail: zhyjiang@tju.edu.cn
Fax: +86 022 23500086
Tel: +86 022 23500086

^b National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, China

^c Department of Bioengineering, School of Chemical Engineering, Hebei University of Technology, Tianjin, China

^d ARC Centre of Excellence for Functional Nanomaterials, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Qld, Australia

Abstract

Inspired by the structural organization of mitochondria and the bioadhesive principle, a simple and versatile approach to construct a multienzyme system is developed. More specifically, the multienzyme system is composed of a polydopamine (PDA) microcapsule scaffold and three spatially separated enzymes. The PDA microcapsules are prepared through the rapid, spontaneous self-polymerization of dopamine on the surface of CaCO₃ microparticle template, followed by dissolution of the template using EDTA. The wall thickness of the microcapsules can be tuned by the dopamine concentration in an aqueous solution. The three enzymes are respectively immobilized through physical encapsulation in the lumen, in situ entrapment within the wall and chemical attachment on the out surface under extremely mild conditions. As an example, a multienzyme system, containing α-amylase, β-amylase and glucosidase, was constructed to convert starch into isomaltooligosaccharide, and the multienzyme system displays higher catalytic activity and enhanced operational stability. The method developed in this study will establish a powerful platform for the facile construction of multienzyme cascade systems.

Green Chemistry

Bioinspired preparation of polydopamine microcapsule for multienzyme system construction†

Abstract

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Bioinspired preparation of polydopamine microcapsule for multienzyme system construction

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