Issue 4, 2012

Impedimetric DNA sensor for detection of Hg2+ and Pb2+

Abstract

An electrochemical DNA sensor based on DNA conformational changes for simultaneous detection of Hg2+ and Pb2+ was reported. The sensor was consisted of a probe strand (DNA), a Pb2+-specific DNAzyme, and a substrate strand contains mercury-specific oligonucleotide (MSO). When Hg2+ and Pb2+ interacted with DNA, the induced conformational changes were tracked by electrochemical impedance spectroscopy (EIS), which led to a decreased RCT. The RCT difference (ΔRCT) was applied to selectively detect Hg2+ and Pb2+ with detection limit of 1 pM and 0.1 pM, respectively. Through using masking agents, such as cysteine (masking Hg2+) and G-DNA (CTG-GGA-GGG-AGG-GAG-GGA) (masking Pb2+), Hg2+ and Pb2+ were simultaneously detected in buffer solution, human serum and river water, respectively.

Graphical abstract: Impedimetric DNA sensor for detection of Hg2+ and Pb2+

Supplementary files

Article information

Article type
Paper
Submitted
08 Nov 2011
Accepted
18 Feb 2012
First published
16 Mar 2012

Anal. Methods, 2012,4, 1036-1040

Impedimetric DNA sensor for detection of Hg2+ and Pb2+

L. Shi, G. Liang, X. Li and X. Liu, Anal. Methods, 2012, 4, 1036 DOI: 10.1039/C2AY05758A

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