Issue 112, 2015
From the journal:

RSC Advances

Micron-sized flower-like Fe3O4@GMA magnetic porous microspheres for lipase immobilization

Zafar Ali,a   Lei Tian, ORCID logo a   Panpan Zhao,a   Baoliang Zhang,a   Ali Nisar,a   Xiangjie Li,a   Hepeng Zhanga  and  Qiuyu Zhang*a  

* Corresponding authors

a The Key Laboratory of Space Applied Physics and Chemistry, School of Science, Northwestern Polytechnical University, Xi’an 710072, China
E-mail: qyzhang@nwpu.edu.cn

Abstract

Flower-like Fe3O4 microspheres prepared by a fast solvothermal method were selected to fabricate micron-sized Fe3O4@glycidyl methacrylate (GMA) magnetic porous microspheres. The magnetic porous microspheres were endowed with appropriate specific surface area (39.54 m2 g−1) and appropriate pore diameter (16 nm), and could serve as a carrier for immobilized lipase. Meanwhile, the prepared magnetic microspheres with high saturation magnetization could realize rapid separations. After those microspheres were aminated and activated, the obtained immobilized lipase possessed high efficiency of immobilization and catalytic activity, with the optimum pH and temperature of 8.0 and 40 °C, respectively. The thermal stability of immobilized lipase was obviously exceeding free lipase.

Graphical abstract: Micron-sized flower-like Fe3O4@GMA magnetic porous microspheres for lipase immobilization

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Article information

DOI
https://doi.org/10.1039/C5RA14524D
Article type
Paper
Submitted
22 Jul 2015
Accepted
07 Oct 2015
First published
09 Oct 2015

RSC Adv., 2015,5, 92449-92455

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Micron-sized flower-like Fe3O4@GMA magnetic porous microspheres for lipase immobilization

Z. Ali, L. Tian, P. Zhao, B. Zhang, A. Nisar, X. Li, H. Zhang and Q. Zhang, RSC Adv., 2015, 5, 92449 DOI: 10.1039/C5RA14524D

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