Issue 120, 2015

Immobilization of laccase on magnetic silica nanoparticles and its application in the oxidation of guaiacol, a phenolic lignin model compound

Abstract

A novel magnetically separable laccase immobilized system was constructed by immobilizing Aspergillus laccase on the core–shell structure of amino-functionalised magnetic silica nanoparticles (AF-MSNPs) via a chemical crosslinking method. A large immobilization capacity (613.5 ± 10.5 mg g−1), activity recovery of 53.4 ± 3.1% and broader pH and temperature profiles than free laccase have been exhibited by the immobilized laccase. Thermal stability, storage stability and operational stability were increased to a great extent. The application of the immobilized system in catalyzing the guaiacol as a phenolic lignin model compound was investigated. The high catalytic efficiency for guaiacol indicated that utilizing immobilized laccase to catalyse lignin is a very promising technology for further applications in the wood industry.

Graphical abstract: Immobilization of laccase on magnetic silica nanoparticles and its application in the oxidation of guaiacol, a phenolic lignin model compound

Article information

Article type
Paper
Submitted
28 Jul 2015
Accepted
11 Nov 2015
First published
19 Nov 2015

RSC Adv., 2015,5, 99439-99447

Author version available

Immobilization of laccase on magnetic silica nanoparticles and its application in the oxidation of guaiacol, a phenolic lignin model compound

J. Hu, B. Yuan, Y. Zhang and M. Guo, RSC Adv., 2015, 5, 99439 DOI: 10.1039/C5RA14982G

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