Sol–gel encapsulation of bacteria: a comparison between alkoxide and aqueous routes

Anne Coiffier; Thibaud Coradin; Cécile Roux; Odile M. M. Bouvet; Jacques Livage

doi:10.1039/B101308O

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Anne Coiffier,^a Thibaud Coradin,^a Cécile Roux,^a Odile M. M. Bouvet^b and Jacques Livage*^a

Author affiliations

* Corresponding authors

^a Chimie de la Matière Condensée, UMR 7574, Université Pierre et Marie Curie, 4 place Jussieu, Paris, France
E-mail: livage@ccr.jussieu.fr

^b Inserm U 389, Institut Pasteur, Paris cedex 15, France

Abstract

The viability of bacteria in the presence of sol–gel reagents has been studied in order to define the best experimental conditions for the sol–gel encapsulation of E. coli. The β-galactosidase activity of these bacteria, trapped in sol–gel silica matrices, was then analyzed. Two routes, using alkoxide and aqueous precursors, have been used and compared. It appears that the aqueous route is less damaging than the alkoxide one. Moreover the aqueous silica matrix appears to slow down the lysis of cell membranes when bacteria are aged without nutrient.

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Article information

DOI: https://doi.org/10.1039/B101308O
Article type: Paper
Submitted: 09 Feb 2001
Accepted: 23 May 2001
First published: 03 Jul 2001

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J. Mater. Chem., 2001,11, 2039-2044

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