Issue 2, 2001
From the journal:

Lab on a Chip

Fully integrated PCR-capillary electrophoresis microsystem for DNA analysis

Eric T. Lagally,a   Charles A. Emrichb  and  Richard A. Mathies*abc  

* Corresponding authors

a UC Berkeley/UC San Francisco Joint Bioengineering Graduate Group, University of California, Berkeley, CA, USA

b UC Berkeley Biophysics Graduate Group, University of California, Berkeley, CA, USA

c Department of Chemistry, University of California, Berkeley, CA, USA
E-mail: rich@zinc.cchem.berkeley.edu

Abstract

A fully integrated genomic analysis microsystem including microfabricated heaters, temperature sensors, and PCR chambers directly connected to capillary electrophoretic separation channels has been constructed. Valves and hydrophobic vents provide controlled and sensorless sample positioning and immobilization into 200 nL PCR chambers. The use of microfabricated heating and temperature sensing elements improves the heating and cooling rates for the PCR reaction to 20 °C s−1. The amplified PCR product, labeled on-column with an intercalating fluorescent dye, is injected into the gel-filled capillary for electrophoretic analysis. Successful sex determination using a multiplex PCR reaction from human genomic DNA is demonstrated in less than 15 min. This device is an important step toward a microfabricated genomic microprocessor for use in forensics and point-of-care molecular medical diagnostics.

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Article information

DOI
https://doi.org/10.1039/B109031N
Article type
Paper
Submitted
04 Oct 2001
Accepted
19 Oct 2001
First published
21 Nov 2001

Lab Chip, 2001,1, 102-107

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Fully integrated PCR-capillary electrophoresis microsystem for DNA analysis

E. T. Lagally, C. A. Emrich and R. A. Mathies, Lab Chip, 2001, 1, 102 DOI: 10.1039/B109031N

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