Issue 2, 2003
From the journal:

Journal of Materials Chemistry

A sol–gel matrix to preserve the viability of encapsulated bacteria

Nadine Nassif,ab   Cécile Roux,a   Thibaud Coradin,a   Marie-Noëlle Rager,c   Odile M. M. Bouvetb  and  Jacques Livage*a  

* Corresponding authors

a Chimie de la Matière Condensée, CNRS UMR 7574, Université Pierre et Marie Curie, 4 place Jussieu, 75252 Paris, France
E-mail: livage@ccr.jussieu.fr

b Unité INSERM 510, Pathogènes et Fonctions des Cellules Epithéliales Polarisées, Faculté de Pharmacie Paris XI, 92296 Châtenay-Malabry, France

c Service de RMN, UMR 7576, ENSCP, 11 rue P. et M. Curie, 75231 Paris, Cedex 05, France

Abstract

E. coli bacteria were encapsulated within silica gels and aged at room temperature in the absence of nutrients. Their viability was studied as a function of time using different viability tests. The plate count technique gives the number of culturable bacteria that remain able to form colonies in the presence of a culture medium. Their metabolic activity toward glycolysis was followed by 14C titration and 13C NMR spectroscopy. Several sol–gel matrices were tested in order to improve the viability of the trapped bacteria. The best results were obtained when encapsulation is performed in the presence of glycerol showing that almost 50% of the bacteria were still able to form metabolites after one month of ageing. Moreover, this study demonstrates that a wide range of viability tests can be adapted for use with cells encapsulated in mineral matrices.

Graphical abstract: A sol–gel matrix to preserve the viability of encapsulated bacteria

About
Cited by
Related
Download options Please wait...

Article information

DOI
https://doi.org/10.1039/B210167J
Article type
Paper
Submitted
16 Oct 2002
Accepted
10 Dec 2002
First published
10 Jan 2003

J. Mater. Chem., 2003,13, 203-208

Permissions

Request permissions

A sol–gel matrix to preserve the viability of encapsulated bacteria

N. Nassif, C. Roux, T. Coradin, M. Rager, O. M. M. Bouvet and J. Livage, J. Mater. Chem., 2003, 13, 203 DOI: 10.1039/B210167J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Spotlight

Advertisements