Issue 1, 2004
From the journal:

Journal of Analytical Atomic Spectrometry

Determination of phosphorus and metals in human brain proteins after isolation by gel electrophoresis by laser ablation inductively coupled plasma source mass spectrometry

J. Sabine Becker,*a   Myroslav Zoriy,a   J. Susanne Becker,b   Carola Pickhardta  and  Michael Przybylskib  

* Corresponding authors

a Central Division of Analytical Chemistry, Research Center Juelich, D-52425 Juelich, Germany
E-mail: s.becker@fz-juelich.de

b Laboratory of Analytical Chemistry, Department of Chemistry, University of Konstanz, D-78457 Konstanz, Germany

Abstract

Phosphorus, sulfur, silicon and metal concentrations (Al, Cu and Zn) were determined in human brain proteins by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) after separation of protein mixtures by two dimensional (2-D) gel electrophoresis. The analysis of phosphorus, silicon and metals in single protein spots in the gel was performed with an optimized microanalytical method using a double-focusing sector field inductively coupled plasma mass spectrometer coupled to a commercial laser ablation system (LA-ICP-MS). Relative ion intensities for P, Si and metals with respect to sulfur in protein spots were determined by LA-ICP-MS. The detection limits for phosphorus and sulfur in protein spots with a silver staining procedure on the 2-D gels were compared with the Coomassie staining technique described previously.

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Article information

DOI
https://doi.org/10.1039/B311274H
Article type
Paper
Submitted
15 Sep 2003
Accepted
24 Oct 2003
First published
08 Dec 2003

J. Anal. At. Spectrom., 2004,19, 149-152

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Determination of phosphorus and metals in human brain proteins after isolation by gel electrophoresis by laser ablation inductively coupled plasma source mass spectrometry

J. S. Becker, M. Zoriy, J. S. Becker, C. Pickhardt and M. Przybylski, J. Anal. At. Spectrom., 2004, 19, 149 DOI: 10.1039/B311274H

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