Issue 20, 2004
From the journal:

Physical Chemistry Chemical Physics

Crystal structure of cyanobacterial photosystem II at 3.2 Å resolution: a closer look at the Mn-cluster†

Jacek Biesiadka,‡*a   Bernhard Loll,‡a   Jan Kern,b   Klaus-Dieter Irrgangb  and  Athina Zouni*b  

* Corresponding authors

a Institute for Chemistry/Crystallography, Free University Berlin, Takustrasse 6, Berlin, Germany
E-mail: jabies@chemie.fu-berlin.de
Fax: +49 30 838 56702
Tel: +49 30 838 56758

b Institute for Chemistry/Max Volmer Laboratory for Biophysical Chemistry, Technical University Berlin, Strasse des 17. Juni 135, Berlin, Germany
E-mail: zouni@phosis1.chem.tu-berlin.de
Fax: +49 30 314 21122
Tel: +49 30 314 25580

Abstract

In the crystal structure of photosystem II (PSII) from the cyanobacterium Thermosynechococcus elongatus at 3.2 Å resolution, several loop regions of the principal protein subunits are now defined that were not interpretable previously at 3.8 Å resolution. The head groups and side chains of the organic cofactors of the electron transfer chain and of antenna chlorophyll a (Chl a) have been modeled, coordinating and hydrogen bonding amino acids identified and the nature of the binding pockets derived. The orientations of these cofactors resemble those of the reaction center from anoxygenic purple bacteria, but differences in hydrogen bonding and protein environment modulate their properties and provide the unique high redox potential (1.17 V) of the primary donor. Coordinating amino acids of manganese cluster, redox-active TyrZ and non-haem Fe2+ have been determined, and an all-trans β-carotene connects cytochrome b-559, ChlZ and primary electron donor (coordinates are available under PDB-code 1W5C).

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Article information

DOI
https://doi.org/10.1039/B406989G
Article type
Paper
Submitted
10 May 2004
Accepted
21 Jul 2004
First published
13 Aug 2004

Phys. Chem. Chem. Phys., 2004,6, 4733-4736

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Crystal structure of cyanobacterial photosystem II at 3.2 Å resolution: a closer look at the Mn-cluster

J. Biesiadka, B. Loll, J. Kern, K. Irrgang and A. Zouni, Phys. Chem. Chem. Phys., 2004, 6, 4733 DOI: 10.1039/B406989G

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