Issue 1, 2005
From the journal:

Lab on a Chip

Apoptotic cell death dynamics of HL60 cells studied using a microfluidic cell trap device

Ana Valero,a   Francisco Merino,a   Floor Wolbers,ab   Regina Luttge,a   Istvan Vermes,b   Helene Anderssonac  and  Albert van den Berg*a  

* Corresponding authors

a BIOS, the Lab-on-a-chip Group, MESA+ Institute for Nanotechnology, University of Twente, PO Box 217, Enschede, The Netherlands
E-mail: a.vandenberg@ewi.utwente.nl
Fax: +31534892287
Tel: +31534892691

b Department of Clinical Chemistry, Medisch SpectrumTwente, Hospital Group, Enschede, The Netherlands

c Royal Institute of Technology, Microsystem Technology, 100 44 Stockholm, Sweden

Abstract

This paper presents the design, fabrication and first results of a microfluidic cell trap device for analysis of apoptosis. The microfluidic silicon-glass chip enables the immobilization of cells and real-time monitoring of the apoptotic process. Induction of apoptosis, either electric field mediated or chemically induced with tumour necrosis factor (TNF-α), in combination with cycloheximide (CHX), was addressed. Exposure of cells to the appropriate fluorescent dyes, FLICA and PI, allows one to discriminate between viable, apoptotic and necrotic cells. The results showed that the onset of apoptosis and the transitions during the course of the cell death cascade were followed in chemically induced apoptotic HL60 cells. For the case of electric field mediated cell death, the distinction between apoptotic and necrotic stage was not clear. This paper presents the first results to analyse programmed cell death dynamics using this apoptosis chip and a first step towards an integrated apoptosis chip for high-throughput drug screening on a single cellular level.

Graphical abstract: Apoptotic cell death dynamics of HL60 cells studied using a microfluidic cell trap device

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Article information

DOI
https://doi.org/10.1039/B415813J
Article type
Paper
Submitted
12 Oct 2004
Accepted
30 Nov 2004
First published
06 Dec 2004

Lab Chip, 2005,5, 49-55

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Apoptotic cell death dynamics of HL60 cells studied using a microfluidic cell trap device

A. Valero, F. Merino, F. Wolbers, R. Luttge, I. Vermes, H. Andersson and A. van den Berg, Lab Chip, 2005, 5, 49 DOI: 10.1039/B415813J

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