Issue 2, 2007
From the journal:

Lab on a Chip

Continuous dielectrophoretic cell separation microfluidic device

Youlan Li,a   Colin Dalton,a   H. John Crabtree,b   Gregory Nilsson†b  and  Karan V. I. S. Kaler*a  

* Corresponding authors

a Biosystems Research and Applications Group, Department of Electrical and Computer Engineering, Schulich School of Engineering, University of Calgary, 2500 University Drive, N.W., Calgary, AB, Canada
E-mail: kaler@ucalgary.ca
Web: http://www.brag.ucalgary.ca
Fax: (403)282-6855
Tel: (403)220-5809

b Micralyne Inc., 1911-94th St., Edmonton, AB, Canada

Abstract

We present a prototype microfluidic device developed for the continuous dielectrophoretic (DEP) fractionation and purification of sample suspensions of biological cells. The device integrates three fully functional and distinct units consisting of an injector, a fractionation region, and two outlets. In the sheath and sample injection ports, the cell sample are hydrodynamically focused into a stream of controlled width; in the DEP fractionation region, a specially shaped nonuniform (isomotive) electric field is synthesized and employed to facilitate the separation, and the sorted cells are then delivered to two sample collection ports. The microfluidic behavior of the injector region was simulated and then experimentally verified. The operation and performance of the device was evaluated using yeast cells as model biological particles. Issues relating to the fabrication and operation of the device are discussed in detail. Such a device takes a significant step towards an integrated lab-on-a-chip device, which could interface/integrate to a number of other on-chip components for the device to undertake the whole laboratory procedure.

Graphical abstract: Continuous dielectrophoretic cell separation microfluidic device

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Article information

DOI
https://doi.org/10.1039/B613344D
Article type
Paper
Submitted
18 Sep 2006
Accepted
10 Nov 2006
First published
01 Dec 2006

Lab Chip, 2007,7, 239-248

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Continuous dielectrophoretic cell separation microfluidic device

Y. Li, C. Dalton, H. J. Crabtree, G. Nilsson and K. V. I. S. Kaler, Lab Chip, 2007, 7, 239 DOI: 10.1039/B613344D

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