Issue 8, 2007
From the journal:

Lab on a Chip

Bead-based immunoassays using a micro-chip flow cytometer†

David Holmes,*ab   Joseph K. She,b   Peter L. Roachb  and  Hywel Morgan*a  

* Corresponding authors

a School of Electronics and Computing Science, University of Southampton, Highfield, Southampton, UK
E-mail: dh2@ecs.soton.ac.uk, hm@ecs.soton.ac.uk
Fax: +44(0)2380 593029
Tel: +44(0)2380 596775

b School of Chemistry, University of Southampton, Highfield, Southampton, UK

Abstract

A microfabricated flow cytometer has been developed for the analysis of micron-sized polymer beads onto which fluorescently labelled proteins have been immobilised. Fluorescence measurements were made on the beads as they flowed through the chip. Binding of antibodies to surface-immobilised antigens was quantitatively assayed using the device. Particles were focused through a detection zone in the centre of the flow channel using negative dielectrophoresis. Impedance measurements of the particles (at 703 kHz) were used to determine particle size and to trigger capture of the fluorescence signal. Antibody binding was measured by fluorescence at single and dual excitation wavelengths (532 nm and 633 nm). Fluorescence compensation techniques were implemented to correct for spectral overspill between optical detection channels. The data from the microfabricated flow cytometer was shown to be comparable to that of a commercial flow cytometer (BD-FACSAria).

Graphical abstract: Bead-based immunoassays using a micro-chip flow cytometer

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Article information

DOI
https://doi.org/10.1039/B707507N
Article type
Paper
Submitted
22 Jan 2007
Accepted
17 May 2007
First published
14 Jun 2007

Lab Chip, 2007,7, 1048-1056

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Bead-based immunoassays using a micro-chip flow cytometer

D. Holmes, J. K. She, P. L. Roach and H. Morgan, Lab Chip, 2007, 7, 1048 DOI: 10.1039/B707507N

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