Issue 1, 2008
From the journal:

Energy & Environmental Science

X-ray crystallography identifies two chloride binding sites in the oxygen evolving centre of Photosystem II

James W. Murray,a   Karim Maghlaoui,a   Joanna Kargul,a   Naoko Ishida,b   Thanh-Lan Lai,b   A. William Rutherford,b   Miwa Sugiura,c   Alain Boussacb  and  James Barber*a  

* Corresponding authors

a Division of Molecular Biosciences, Imperial College London, London, UK
E-mail: j.barber@imperial.ac.uk
Fax: +44 20 759 45267
Tel: +44 20 759 45266

b iBiTec-S, URA CNRS 2096, CEA Saclay, Gif sur Yvette, France

c Cell-Free Science and Technology Research Center, Ehime University, Bunkyo-cho, Matsuyama Ehime, Japan

Abstract

Bromide anomalous X-ray diffraction analyses have been used to locate chloride binding sites in the vicinity of the water splitting/oxygen evolving centre (OEC) of Photosystem II. Three-dimensional crystals of PSII from Thermosynechococcus elongatus were grown from (i) isolated PSII crystals infiltrated with bromide or (ii) PSII obtained from cells cultured in a medium in which the chloride content was totally replaced by bromide. In either case, the anomalous diffraction yielded the same result, the existence of two bromide binding sites in the vicinity of the OEC. Neither are in the first coordination sphere of the Mn and Ca ions which form the catalytic centre of the OEC, being about 6 to 7 Å from the metal-cluster. Site 1 is located close to the side chain nitrogen of D2-K317 and the backbone nitrogen of D1-Glu333 while Site 2 is adjacent to backbone nitrogens of CP43-Glu354 and D1-Asn338. Their positioning close to postulated hydrophilic channels may suggest a role in proton removal from, or substrate access to, the OEC.

Graphical abstract: X-ray crystallography identifies two chloride binding sites in the oxygen evolving centre of Photosystem II

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Article information

DOI
https://doi.org/10.1039/B810067P
Article type
Paper
Submitted
13 Jun 2008
Accepted
18 Jun 2008
First published
02 Jul 2008

Energy Environ. Sci., 2008,1, 161-166

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X-ray crystallography identifies two chloride binding sites in the oxygen evolving centre of Photosystem II

J. W. Murray, K. Maghlaoui, J. Kargul, N. Ishida, T. Lai, A. W. Rutherford, M. Sugiura, A. Boussac and J. Barber, Energy Environ. Sci., 2008, 1, 161 DOI: 10.1039/B810067P

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