Issue 14, 2009
From the journal:

Lab on a Chip

A microfluidic 3D hepatocyte chip for drug toxicity testing†

Yi-Chin Toh,a   Teck Chuan Lim,a   Dean Tai,a   Guangfa Xiao,abc   Danny van Noort*a  and  Hanry Yu*abdefgh  

* Corresponding authors

a Institute of Bioengineering and Nanotechnology, A*STAR, The Nanos, #04-01, 31 Biopolis Way, Singapore, Singapore
Fax: +65 6478 9080
Tel: +65 6824 7103

b Department of Physiology, Yong Loo Lin School of Medicine, #03-03, 2 Medical Drive, Singapore, Singapore

c Department of General Surgery, XiangYa Hospital, Central-South University, Changsha, Hunan, China

d Singapore-MIT Alliance, E4-04-10, 4 Engineering Drive 3, Singapore, Singapore

e NUS Tissue-Engineering Programme, DSO Labs, National University of Singapore, Singapore, Singapore

f Department of Haematology-Oncology, National University Hospital, Singapore, Singapore

g Graduate Program in Bioengineering, NUS Graduate School for Integrative Sciences and Engineering, Centre for Life Sciences (CeLS), #05-01, 28 Medical Drive, Singapore, Singapore

h Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA

Abstract

We have developed a microfluidic 3D hepatocyte chip (3D HepaTox Chip) for in vitro drug toxicity testing to predict in vivo drug hepatotoxicity. The 3D HepaTox Chip is based on multiplexed microfluidic channels where a 3D microenvironment is engineered in each channel to maintain the hepatocytes' synthetic and metabolic functions. The multiplexed channels allow for simultaneous administration of multiple drug doses to functional primary hepatocytes while an incorporated concentration gradient generator enables the in vitro dose-dependent drug responses to predict in vivo hepatotoxicity. The IC50 values of 5 model drugs derived from the dose-dependent on-chip testing correlate well with the reported in vivo LD50 values. The 3D HepaTox Chip can be integrated with on-chip sensors and actuators as the next generation cell-based on-chip drug testing platform.

Graphical abstract: A microfluidic 3D hepatocyte chip for drug toxicity testing

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Article information

DOI
https://doi.org/10.1039/B900912D
Article type
Paper
Submitted
15 Jan 2009
Accepted
01 Apr 2009
First published
20 Apr 2009

Lab Chip, 2009,9, 2026-2035

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A microfluidic 3D hepatocyte chip for drug toxicity testing

Y. Toh, T. C. Lim, D. Tai, G. Xiao, D. van Noort and H. Yu, Lab Chip, 2009, 9, 2026 DOI: 10.1039/B900912D

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