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Sensitized formation of oxidatively generated damage to cellular DNA by UVA radiation

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Abstract

The survey is aimed at critically reviewing information on the UVA-mediated oxidative reactions to cellular components with emphasis on DNA as the result of mostly photosensitized pathways. It appears clearly that UVA radiation is relatively much more efficient than UVB photons in inducing oxidative processes. The main UVA-induced oxidative degradation pathways of DNA are reported and discussed mechanistically. They are mostly rationalized in terms of a major contribution of singlet molecular oxygen (1O2) and to a lesser extent of hydroxyl radical (·OH), that in the latter case originates from Fenton-type reactions. This leads to the predominant formation of 8-oxo-7,8-dihydroguanine together with smaller amounts of oxidized pyrimidine bases and DNA strand breaks in UVA-irradiated cells.

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Abbreviations

CPD:

Cyclobutadipyrimidines

CHO:

Chinese hamster ovary

Endo:

III Endonuclease III

DHPN:

N,N′-Di(2,3-dihydroxypropyl)-1,4-naphthalenedipropanamide

FapyGua:

2,6-Diamino-4-hydroxy-5-formamidopyrimidine

Fpg:

Formamidopyrimidine DNA N-glycosylase

HPLC-ECD:

High performance liquid chromatography associated with electrochemical detection

HPLC-MS/MS:

High performance liquid chromatography associated with tandem mass spectrometry

MRM:

Multiple reaction monitoring

5-OHCyt:

5-Hydroxycytosine

8-oxoGua:

8-Oxo-7,8-dihydroguanine

8-oxodGuo:

8-Oxo-7,8-dihydro-2′-deoxyguanosine

6–4PP:

Pyrimidine (6–4) pyrimidone

Tg:

5,6-Dihydroxy-5,6-dihydrothymine.

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  1. aLaboratoire Lésions des Acides Nucléiques; SCIB-UMR-E no 3 (CEA/UJF), Institut Nanosciences et Cryogénie; CEA/Grenoble, 38054, Grenoble, France

    Jean Cadet, Thierry Douki & Jean-Luc Ravanat

  2. Departamento de Bioquímica, Instituto de Química, Universidade de ão Paulo, CP26077, CEP, 05513-970, ão Paulo, SP, Brazil

    Paolo Di Mascio

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  1. Jean Cadet
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Correspondence to Jean Cadet.

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† This article was published as part of the themed issue in honour of Esther Oliveros.

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Cadet, J., Douki, T., Ravanat, JL. et al. Sensitized formation of oxidatively generated damage to cellular DNA by UVA radiation. Photochem Photobiol Sci 8, 903–911 (2009). https://doi.org/10.1039/b905343n

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