Issue 10, 2009
From the journal:

Analyst

Cell separation by an aqueous two-phase system in a microfluidic device

Masatoshi Tsukamoto,a   Shu Taira,a   Shohei Yamamura,b   Yasutaka Morita,c   Naoki Nagatani,d   Yuzuru Takamuraa  and  Eiichi Tamiya*e  

* Corresponding authors

a School of Materials Science, Japan Advanced Institute of Science and Technology (JAIST), Asahidai, Nomi, Ishikawa 923-1211, Japan

b Health Technology Research Center, Advanced Industrial Science and Technology Shikoku, 2217-14 Hayashi-cho, Takamatsu, Kagawa 761-0395, Japan

c Faculty of Human-oriented Science and Engineering, Kinki University, 11-6 Kashiwanomori, Iizuka, Fukuoka 820-8555, Japan

d Department of Biotechnology and Applied Chemistry, Faculty of Engineering, Okayama University of Science, 1-1 Ridai-cho, Okayama 700-0005, Japan

e Department of Applied Physics, Graduate School of Engineering, 2-1 Yamadaoka, Suita, Osaka, Japan
E-mail: tamiya@ap.eng.osaka-u.ac.jp
Fax: +81-6-6879-4087
Tel: +81-6-6879-4087

Abstract

We generated an aqueous two-phase laminar flow in a microfluidic chip and used the system to isolate leukocyte and erythrocyte cells from whole blood cells. The microfluidic system reduced the effect of gravity in the aqueous two-phase system (ATPS). Poly(ethylene glycol) (PEG) and dextran (Dex) solutions were used as the two phases, and the independent flow rates of the solutions were both 2 µL/min. When hydrophobic and hydrophilic polystyrene beads were introduced into the microfluidic device, the hydrophilic beads moved to the Dex layer and the hydrophobic beads to the interface between the two phases. In the case of living cells, Jurkat cells and erythrocytes moved more efficiently to the PEG and Dex layers, respectively, than they move in a conventional ATPS. When whole blood cells were inserted into the microfluidic chip, leukocytes could be separated from erythrocytes because erythrocytes moved to the Dex layer while leukocytes remained outside of this layer in the microfluidic system. The reported microfluidic chip for the whole blood cell separation can effectively be integrated into a Micro Total Analysis System designed for cell-based clinical, forensic, and environmental analyses.

Graphical abstract: Cell separation by an aqueous two-phase system in a microfluidic device

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Article information

DOI
https://doi.org/10.1039/B909597G
Article type
Communication
Submitted
27 May 2009
Accepted
03 Aug 2009
First published
07 Aug 2009

Analyst, 2009,134, 1994-1998

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Cell separation by an aqueous two-phase system in a microfluidic device

M. Tsukamoto, S. Taira, S. Yamamura, Y. Morita, N. Nagatani, Y. Takamura and E. Tamiya, Analyst, 2009, 134, 1994 DOI: 10.1039/B909597G

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