Issue 12, 2010
From the journal:

Lab on a Chip

Validation of a blood plasma separation system by biomarker detection

Maïwenn Kersaudy-Kerhoas,*a   Deirdre M. Kavanagh,a   Resham S. Dhariwal,a   Colin J. Campbellbc  and  Marc P. Y. Desmullieza  

* Corresponding authors

a MIcroSystems Engineering Centre (MISEC), School of Engineering & Physical Sciences, Heriot-Watt University, Earl Mountbatten Building, Edinburgh, United Kingdom
E-mail: mk87@hw.ac.uk
Fax: +44 131 451 4155
Tel: +44 131 451 4148

b Division of Pathway Medicine, University of Edinburgh Medical School, Chancellor's Building, 49 Little France Crescent, Edinburgh, United Kingdom
E-mail: colin.campbell@ed.ac.uk
Fax: +44 131 242 6244
Tel: +44 131 242 6242

c School of Chemistry, University of Edinburgh, Joseph Black building, West Mains road, Edinburgh, United Kingdom
E-mail: colin.campbell@ed.ac.uk
Fax: +44 131 650 6453
Tel: +44 131 650 7546

Abstract

A microfluidic system was developed for blood plasma separation at high flow rate. This system uses only hydrodynamic forces to separate plasma from whole blood. The microfluidic network features a series of constrictions and bifurcations to enhance the product yield and purity. A maximum purity efficiency of 100% is obtained on blood with entrance hematocrit level up to 30% with a flow rate of 2 mL h−1. Flow cytometry was performed on the extracted plasma to evaluate the separation efficiency and to assess cell damage. A core target of this study was the detection of cell-free DNA from the on-chip extracted plasma. To this effect, PCR was successfully carried out off-chip on the cell-free DNA present in the plasma extracted on-chip. A house-keeping gene sequence (GAPDH) was amplified without the need for a purification after the separation, thereby showing the high quality of the plasma sample. The resulting data suggests that the system can be used as a preliminary module of a total analysis system for cell-free DNA detection in human plasma.

Graphical abstract: Validation of a blood plasma separation system by biomarker detection

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Article information

DOI
https://doi.org/10.1039/B926834K
Article type
Paper
Submitted
22 Dec 2009
Accepted
03 Mar 2010
First published
31 Mar 2010

Lab Chip, 2010,10, 1587-1595

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Validation of a blood plasma separation system by biomarker detection

M. Kersaudy-Kerhoas, D. M. Kavanagh, R. S. Dhariwal, C. J. Campbell and M. P. Y. Desmulliez, Lab Chip, 2010, 10, 1587 DOI: 10.1039/B926834K

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