Issue 36, 2010
From the journal:

Physical Chemistry Chemical Physics

Fluorescence correlation spectroscopy reveals strong fluorescence quenching of FITC adducts on PEGylated gold nanoparticles in water and the presence of fluorescent aggregates of desorbed thiolate ligands†

Matthieu Loumaigne,a   Raïssa Praho,bc   Daniele Nutarelli,a   Martinus H. V. Werts*bc  and  Anne Débarre*a  

* Corresponding authors

a CNRS, Laboratoire Aimé Cotton (UPR 3321), Université Paris Sud, Bâtiment 505, F-91405 Orsay, France
E-mail: anne.debarre@lac.u-psud.fr

b Ecole Normale Supérieure de Cachan/Bretagne, SATIE (UMR 8029), Campus de Ker Lann, F-35170 Bruz, France
E-mail: martinus.werts@bretagne.ens-cachan.fr

c CNRS, SATIE (UMR 8029), ENS Cachan/Bretagne, Campus de Ker Lann, F-35170 Bruz, France

Abstract

Colloidal gold particles functionalised with oligoethylene-glycolated disulfide ligands and fluorescent moieties derived from fluorescein isothiocyanate (FITC) have been prepared and studied in aqueous suspension using fluorescence correlation spectroscopy (FCS). FCS probes the dynamics of the particles at the single object level, and reveals the desorption of fluorescent ligands which subsequently aggregate into larger (slower diffusing) objects. Cross-correlation spectroscopy of the FITC fluorescence and the Rayleigh-Mie scattering (RM-FCCS) of the gold cores shows that the only detectable fluorescent objects are free ligands and aggregates not associated with a gold particle. The fluorescence of bound fluorophores is quenched making their fluorescence too weak to be detected. FCS and RM-FCCS are useful tools for characterising functionalised noble metal particles in solution, under conditions similar to those used in optical bio-imaging. Desorption of thiolates from gold nanoparticles needs to be taken into account when working with these materials at low concentration.

Graphical abstract: Fluorescence correlation spectroscopy reveals strong fluorescence quenching of FITC adducts on PEGylated gold nanoparticles in water and the presence of fluorescent aggregates of desorbed thiolate ligands

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Article information

DOI
https://doi.org/10.1039/C004167J
Article type
Paper
Submitted
11 Mar 2010
Accepted
20 May 2010
First published
28 Jul 2010

Phys. Chem. Chem. Phys., 2010,12, 11004-11014

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Fluorescence correlation spectroscopy reveals strong fluorescence quenching of FITC adducts on PEGylated gold nanoparticles in water and the presence of fluorescent aggregates of desorbed thiolate ligands

M. Loumaigne, R. Praho, D. Nutarelli, M. H. V. Werts and A. Débarre, Phys. Chem. Chem. Phys., 2010, 12, 11004 DOI: 10.1039/C004167J

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