Issue 20, 2010
From the journal:

Lab on a Chip

Ultrasound-controlled cell aggregation in a multi-well chip†

Bruno Vanherberghen,‡a   Otto Manneberg,§‡a   Athanasia Christakou,a   Thomas Frisk,a   Mathias Ohlin,a   Hans M. Hertz,a   Björn Önfelt*ab  and  Martin Wiklund*a  

* Corresponding authors

a Department of Applied Physics, Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden
E-mail: martin.wiklund@biox.kth.se, bjorn.onfelt@ki.se

b Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden

Abstract

We demonstrate a microplate platform for parallelized manipulation of particles or cells by frequency-modulated ultrasound. The device, consisting of a silicon-glass microchip and a single ultrasonic transducer, enables aggregation, positioning and high-resolution microscopy of cells distributed in an array of 100 microwells centered on the microchip. We characterize the system in terms of temperature control, aggregation and positioning efficiency, and cell viability. We use time-lapse imaging to show that cells continuously exposed to ultrasound are able to divide and remain viable for at least 12 hours inside the device. Thus, the device can be used to induce and maintain aggregation in a parallelized fashion, facilitating long-term microscopy studies of, e.g., cell–cell interactions.

Graphical abstract: Ultrasound-controlled cell aggregation in a multi-well chip

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Article information

DOI
https://doi.org/10.1039/C004707D
Article type
Paper
Submitted
30 Mar 2010
Accepted
30 Jul 2010
First published
31 Aug 2010

Lab Chip, 2010,10, 2727-2732

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Ultrasound-controlled cell aggregation in a multi-well chip

B. Vanherberghen, O. Manneberg, A. Christakou, T. Frisk, M. Ohlin, H. M. Hertz, B. Önfelt and M. Wiklund, Lab Chip, 2010, 10, 2727 DOI: 10.1039/C004707D

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