Issue 11, 2010
From the journal:

Analyst

Phenol biosensor based on hydrogel microarrays entrapping tyrosinase and quantum dots

Eunji Jang,a   Kyung Jin Son,a   Bumsang Kimb  and  Won-Gun Koh*a  

* Corresponding authors

a Department of Chemical and Biomolecular Engineering, Yonsei University, 134 Sinchon-Dong, Seodaemoon-Gu, Seoul, Republic of Korea
E-mail: wongun@yonsei.ac.kr
Fax: +82-2-312-6401
Tel: +82-2-2123-5755

b Department of Chemical Engineering, Hongik University, 72-1 Sangsu-dong Mapo-gu, Seoul, Korea

Abstract

This paper describes the use of microarray-based biosensor system for the determination of phenol. Microarrays based on poly(ethylene glycol)(PEG) hydrogel were prepared by photopatterning of a solution containing PEG diacrylate (PEG-DA), photoinitiator, tyrosinase, and CdSe/ZnS quantum dots (QDs). During photo-induced crosslinking, tyrosinase and QDs were entrapped within the hydrogel microarrays, making the hydrogel microarray fluorescent and responsive to phenol. The entrapped tyrosinase could carry out enzyme-catalyzed oxidation of phenol to produce quinones, which subsequently quenched the fluorescence of QDs within hydrogel microarray. The fluorescence intensity of the hydrogel microarrays decreased linearly according to phenol concentration and the detection limit of this system was found to be 1.0 μM. The microarray system presented in this study could be combined with a microfluidic device as an initial step to create a phenol-detecting “micro-total-analysis-system (μ-TAS)”.

Graphical abstract: Phenol biosensor based on hydrogel microarrays entrapping tyrosinase and quantum dots

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Article information

DOI
https://doi.org/10.1039/C0AN00353K
Article type
Paper
Submitted
26 May 2010
Accepted
09 Aug 2010
First published
20 Sep 2010

Analyst, 2010,135, 2871-2878

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Phenol biosensor based on hydrogel microarrays entrapping tyrosinase and quantum dots

E. Jang, K. J. Son, B. Kim and W. Koh, Analyst, 2010, 135, 2871 DOI: 10.1039/C0AN00353K

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