Issue 8, 2011
From the journal:

Analyst

Sensitive electrochemical detection of superoxide anion using gold nanoparticles distributed poly(methyl methacrylate)–polyaniline core–shell electrospun composite electrode

Padmanabhan Santhosh,ab   Kalayil Manian Manesh,a   Se-Hee Lee,a   Sivaperumal Uthayakumar,b   Anantha Iyengar Gopalana  and  Kwang-Pill Lee*a  

* Corresponding authors

a Department of Chemistry Graduate School, Kyungpook National University, Daegu, South Korea
E-mail: kplee@knu.ac.kr
Fax: +82 53 952 8104
Tel: +82 53 950 5901

b Max-Planck-Institute for Solid State Research, Heisenbergstrasse 1, Stuttgart, Germany

Abstract

In the present communication, a novel composite nanofibrous electrode is developed for the detection of superoxide anion (O2˙−) in phosphate buffered saline (PBS). The composite fiber electrode is fabricated by dispersing gold nanoparticles onto poly(methyl methacrylate) (PMMA)–polyaniline (PANI) core–shell electrospun nanofibers. The constructed architecture is proven to be a favorable environment for the immobilization of the enzyme, superoxide dismutase (SOD). Direct electron transfer is achieved between SOD and the electrode with an electron transfer rate constant of 8.93 s−1. At an applied potential of +300 mV, PMMA/PANI–Aunano/SOD–ESCFM shows highly sensitive detection of O2˙−. In addition to this, quantification of different activities of SOD is realized at PMMA/PANI–Aunano/SOD–ESCFM. These analytical features offer great potential for construction of the third-generation O2˙− biosensor.

Graphical abstract: Sensitive electrochemical detection of superoxide anion using gold nanoparticles distributed poly(methyl methacrylate)–polyaniline core–shell electrospun composite electrode

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Article information

DOI
https://doi.org/10.1039/C0AN00616E
Article type
Communication
Submitted
09 Aug 2010
Accepted
24 Jan 2011
First published
15 Feb 2011

Analyst, 2011,136, 1557-1561

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Sensitive electrochemical detection of superoxide anion using gold nanoparticles distributed poly(methyl methacrylate)–polyaniline core–shell electrospun composite electrode

P. Santhosh, K. M. Manesh, S. Lee, S. Uthayakumar, A. I. Gopalan and K. Lee, Analyst, 2011, 136, 1557 DOI: 10.1039/C0AN00616E

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