Issue 12, 2010
From the journal:

Analytical Methods

Detection of influenza viruses by a waveguide-mode sensor†

Subash C. B. Gopinath,*a   Koichi Awazu*a  and  Makoto Fujimakia  

* Corresponding authors

a Photonic Research Institute, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, Ibaraki, Japan
E-mail: gopi-subashchandrabose@aist.go.jp, k.awazu@aist.go.jp

Abstract

Methods are demonstrated for antibody-based detection of influenza viruses using the monolithic-sensing-plate technology of an evanescent-field-coupled waveguide-mode sensor. To facilitate the detection of viruses at lower concentrations, a strategy of complexing the virus with Coomassie Brilliant Blue dye was used to amplify the signal. By applying this method, hemagglutinins from avian influenza virus (H5N1) and human influenza virus (A/Panama/2007/1999) were detected. An intact human influenza virus (A/Brisbane/59/2007) was also detected. The method is suitable for the detection of virulent viruses that may emerge at various stages of evolution.

Graphical abstract: Detection of influenza viruses by a waveguide-mode sensor

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Article information

DOI
https://doi.org/10.1039/C0AY00491J
Article type
Paper
Submitted
10 Aug 2010
Accepted
22 Sep 2010
First published
26 Oct 2010

Anal. Methods, 2010,2, 1880-1884

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Detection of influenza viruses by a waveguide-mode sensor

S. C. B. Gopinath, K. Awazu and M. Fujimaki, Anal. Methods, 2010, 2, 1880 DOI: 10.1039/C0AY00491J

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