Issue 16, 2011
From the journal:

Analyst

Preparation and evaluation of a phenylboronate affinity monolith for selective capture of glycoproteins by capillary liquid chromatography

Zi An Lin,*a   Ji Lei Pang,a   Yao Lin,a   Hui Huang,a   Zong Wei Cai,b   Lan Zhang*a  and  Guo Nan Chena  

* Corresponding authors

a Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian, China
E-mail: zianlin@fzu.edu.cn, zlan@fzu.edu.cn
Fax: +86-591-28866135
Tel: +86-591-28866132

b Department of Chemistry, Hong Kong Baptist University, Hong Kong, SAR, P. R. China

Abstract

A phenylboronate affinity monolith was prepared and applied to the selective capture of glycoproteins from unfractionated protein mixtures. The monolith was synthesized in a 100 μm i.d capillary by an in situpolymerization procedure using a pre-polymerization mixture consisting of 4-vinylphenylboronic acid (VPBA) as functional monomer, ethylene dimethacrylate (EDMA) as crosslinker, diethylene glycol and ethylene glycol as binary porogenic solvents, and azobisisobutyronitrile (AIBN) as initiator. The prepared monolith was characterized in terms of the morphology, pore property, and recognition property. The selectivity and dynamic binding capacity were evaluated by using standard glycoproteins and nonglycoproteins as model proteins. The chromatographic results demonstrated that the phenylboronate affinity monolith had higher selectivity and binding capacity for glycoprotein than nonglycoprotein. The resulting phenylboronate affinity monolith was used as the sorbent for in-tube solid phase microextraction (in-tube SPME), and the extraction performance of the monolith was assessed by capture of ovalbumin from egg white sample.

Graphical abstract: Preparation and evaluation of a phenylboronate affinity monolith for selective capture of glycoproteins by capillary liquid chromatography

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Article information

DOI
https://doi.org/10.1039/C1AN15180K
Article type
Paper
Submitted
05 Mar 2011
Accepted
28 May 2011
First published
08 Jul 2011

Analyst, 2011,136, 3281-3288

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Preparation and evaluation of a phenylboronate affinity monolith for selective capture of glycoproteins by capillary liquid chromatography

Z. A. Lin, J. L. Pang, Y. Lin, H. Huang, Z. W. Cai, L. Zhang and G. N. Chen, Analyst, 2011, 136, 3281 DOI: 10.1039/C1AN15180K

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