Issue 12, 2011
From the journal:

Analyst

Characterisation of chondrogenic differentiation of human mesenchymal stem cells using synchrotron FTIR microspectroscopy

Chirapond Chonanant,†a   Nichada Jearanaikoon,†b   Chanvit Leelayuwat,a   Temduang Limpaiboon,a   Mark J. Tobin,c   Patcharee Jearanaikoon*a  and  Philip Heraud*de  

* Corresponding authors

a Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand
E-mail: patjea@kku.ac.th
Fax: +66 4334 7482
Tel: +66 4334 7482

b Synchrotron Light Research Institute (Public Organization), Nakhon Ratchasima, Thailand

c Australian Synchrotron, Clayton, Victoria, Australia

d Monash Immunology and Stem Cell Laboratories, Monash University, Victoria, Australia
E-mail: Phil.Heraud@med.monash.edu.au
Fax: +61 3 9905 4597
Tel: +61 3 9905 4597

e Centre for Biospectroscopy, School of Chemistry, Monash University, Victoria, Australia

Abstract

A major limiting factor in stem cell therapy is the accurate identification of the differentiation state of cells destined for transplantation. This study aimed to evaluate the potential of synchrotron radiation Fourier transform infrared (SR-FTIR) microspectroscopy as a novel technique to probe the differentiation state of human mesenchymal stem cells (hMSCs) to chondrocytes over a period of 7, 14 and 21 days of induction. The chondrogenic markers were determined using reverse transcription polymerase chain reaction, histology and immunohistochemistry. The changes of average spectra located near 1338–1230 and 1175–960 cm−1 indicated increased levels of collagen and aggrecan, respectively, in chondrocyte-induced hMSCs compared with control cells. Classification of independent test spectra using partial least squares discriminant analysis (PLS-DA) could distinguish control and chondrocyte-induced cells with 100% accuracy. We conclude that the SR-FTIR microspectroscopy technique is sensitive for monitoring the differentiation state of stem cells under chondrogenic induction particularly at an early stage. It provides biochemical information that is complimentary to that obtained from conventional techniques, and may give more unambiguous results particularly at the very early stage of cellular differentiation. In addition, the spectroscopic approach is more straightforward, non-destructive and requires less sample preparation compared with the conventional methodologies.

Graphical abstract: Characterisation of chondrogenic differentiation of human mesenchymal stem cells using synchrotron FTIR microspectroscopy

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Article information

DOI
https://doi.org/10.1039/C1AN15182G
Article type
Paper
Submitted
05 Mar 2011
Accepted
24 Mar 2011
First published
28 Apr 2011

Analyst, 2011,136, 2542-2551

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Characterisation of chondrogenic differentiation of human mesenchymal stem cells using synchrotron FTIR microspectroscopy

C. Chonanant, N. Jearanaikoon, C. Leelayuwat, T. Limpaiboon, M. J. Tobin, P. Jearanaikoon and P. Heraud, Analyst, 2011, 136, 2542 DOI: 10.1039/C1AN15182G

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